Mapping of pain circuitry in early post-natal development using manganese-enhanced MRI in rats.
Mapping of pain circuitry in early post-natal development using manganese-enhanced MRI in rats.
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DOI：
10.1016/j.neuroscience.2017.03.052
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发表时间：
2017-06-03
期刊：
影响因子：
3.3
通讯作者：
Barr GA
中科院分区：
文献类型：
--
作者：
Sperry MM;Kandel BM;Wehrli S;Bass KN;Das SR;Dhillon PS;Gee JC;Barr GA
作为必要医疗护理的一部分，早产或患病的足月婴儿会接受许多有害程序。尽管我们知道人类婴儿会因此类操作而表现出神经变化，但我们对疼痛激活的感觉或情感大脑回路知之甚少。在啮齿动物模型中，重点是脊髓，最近的重点是中脑和髓质。本研究使用锰增强磁共振成像（MEMRI）评估大脑回路的激活。锰是一种顺磁造影剂，通过活跃的突触和轴突运输，通过在 12 天大的幼鼠后爪注射稀释福尔马林来测量锰的摄取量，在这个年龄，老鼠开始表现出厌恶学习的年龄，大致相当于足月的人类婴儿。福尔马林在这个年龄诱导了广泛报道的双相反应，并诱导了对其注射相关线索的条件性厌恶，从而证明了刺激的厌恶性。形态测量分析、结构方程建模和共表达分析表明边缘和感觉路径被激活，其中最突出的是前额叶和前扣带皮层、伏核、杏仁核、下丘脑、一些脑干结构和小脑。因此，成年大鼠中由疼痛激活的感觉和情感回路也可以由 12 日龄幼鼠的有害刺激激活。
Premature or ill full-term infants are subject to a number of noxious procedures as part of their necessary medical care. Although we know that human infants show neural changes in response to such procedures, we know little of the sensory or affective brain circuitry activated by pain. In rodent models, the focus has been on spinal cord and, more recently, midbrain and medulla. The present study assesses activation of brain circuits using manganese-enhanced magnetic resonance imaging (MEMRI). Uptake of manganese, a paramagnetic contrast agent that is transported across active synapses and along axons, was measured in response to a hindpaw injection of dilute formalin in 12-day-old rat pups, the age at which rats begin to show aversion learning and which is roughly the equivalent of full-term human infants. Formalin induced the well-reported biphasic response at this age and induced a conditioned aversion to cues associated with its injection, thus demonstrating the aversiveness of the stimulation. Morphometric analyses, structural equation modeling and co-expression analysis showed that limbic and sensory paths were activated, the most prominent of which were the prefrontal and anterior cingulate cortices, nucleus accumbens, amygdala, hypothalamus, several brainstem structures, and the cerebellum. Therefore, both sensory and affective circuits, which are activated by pain in the adult, can also be activated by noxious stimulation in 12-day-old rat pups.
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影响因子：
5.7
作者：
Aoki, I;Wu, YJL;Koretsky, AP
通讯作者：
Koretsky, AP
影响因子：
3.4
作者：
BARR, GA;WANG, SN;CARDEN, S
通讯作者：
CARDEN, S
影响因子：
4
作者：
Barr, Gordon A.
通讯作者：
Barr, Gordon A.
DOI：
10.1196/annals.1301.009
发表时间：
2003-01-01
期刊：
ROOTS OF MENTAL ILLNESS IN CHILDREN
影响因子：
--
作者：
Connor, DF;Doerfler, LA;Melloni, RH
通讯作者：
Melloni, RH
影响因子：
5.7
作者：
Chan, Kevin C.;Xing, Kyle K.;Wu, Ed X.
通讯作者：
Wu, Ed X.