Maternal Haploid, a Metalloprotease Enriched at the Largest Satellite Repeat and Essential for Genome Integrity in Drosophila Embryos
Maternal Haploid, a Metalloprotease Enriched at the Largest Satellite Repeat and Essential for Genome Integrity in Drosophila Embryos
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母本单倍体，一种富含最大卫星重复序列的金属蛋白酶，对果蝇胚胎基因组完整性至关重要
DOI：
10.1534/genetics.117.200949
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发表时间：
2017-06
期刊：
影响因子：
3.3
通讯作者：
Rong Yikang S.
中科院分区：
文献类型：
--
作者：
Tang Xiaona;Cao Jinguo;Zhang Liang;Huang Yingzi;Zhang Qianyi;Rong Yikang S.
在受精过程中，父本基因组整合到合子中需要染色质重塑。果蝇中的母本单倍体（mh）突变影响了这一过程，并导致形成没有父本基因组的单倍体胚胎。mh编码果蝇SPRTN的同源物，SPRTN是一种保守的蛋白酶，对分解DNA-蛋白质交联产物至关重要。在这里，我们的特点MH在基因组维护中的作用。目前还不清楚MH如何在受精过程中保护父本基因组，特别是根据我们的发现，MH是在受精卵形成过程中存在于两个亲本原核。我们发现，在mh突变体胚胎中的母体染色体经历不稳定的父基因组的情况下，这表明，MH通常需要在胚胎发生过程中的染色体稳定性。这与我们的发现一致，即MH在整个细胞周期中大量存在于染色质上。值得注意的是，在间期期间，MH在359-bp卫星重复序列处显著富集，没有MH，其变得不稳定。MH在359个重复序列处的这种动态定位和特异性富集类似于拓扑异构酶2（Top2），表明MH调节Top2，可能作为用于解析Top2-DNA中间体的蛋白酶。我们建议，母亲MH删除蛋白质，特别是丰富的精子染色质。如果没有这种功能，父亲的染色体就会急剧丢失。这种模式的父系染色质重塑可能是保守的，果蝇mh突变体的独特表型代表了一个难得的机会，以获得深入了解的过程中，一直难以研究。
The incorporation of the paternal genome into the zygote during fertilization requires chromatin remodeling. The maternal haploid (mh) mutation in Drosophila affects this process and leads to the formation of haploid embryos without the paternal genome. mh encodes the Drosophila homolog of SPRTN, a conserved protease essential for resolving DNA–protein cross-linked products. Here we characterize the role of MH in genome maintenance. It is not understood how MH protects the paternal genome during fertilization, particularly in light of our finding that MH is present in both parental pronuclei during zygote formation. We showed that maternal chromosomes in mh mutant embryos experience instabilities in the absence of the paternal genome, which suggests that MH is generally required for chromosome stability during embryogenesis. This is consistent with our finding that MH is abundantly present on chromatin throughout the cell cycle. Remarkably, MH is prominently enriched at the 359-bp satellite repeats during interphase, which becomes unstable without MH. This dynamic localization and specific enrichment of MH at the 359 repeats resemble that of Topoisomerase 2 (Top2), suggesting that MH regulates Top2, possibly as a protease for the resolution of Top2-DNA intermediates. We propose that maternal MH removes proteins specifically enriched on sperm chromatin. In the absence of that function, paternal chromosomes are precipitously lost. This mode of paternal chromatin remodeling is likely conserved and the unique phenotype of the Drosophila mh mutants represents a rare opportunity to gain insights into the process that has been difficult to study.
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影响因子：
16.6
作者：
Maskey, Reeja S.;Kim, Myoung Shin;Baker, Darren J.;Childs, Bennett;Malureanu, Liviu A.;Jeganathan, Karthik B.;Machida, Yuka;van Deursen, Jan M.;Machida, Yuichi J.
通讯作者：
Machida, Yuichi J.
影响因子：
8.8
作者：
Doyen, Cecile M.;Chalkley, Gillian E.;Verrijzer, C. Peter
通讯作者：
Verrijzer, C. Peter
影响因子：
14.9
作者：
Kim MS;Machida Y;Vashisht AA;Wohlschlegel JA;Pang YP;Machida YJ
通讯作者：
Machida YJ
DOI：
10.1073/pnas.0902707106
发表时间：
2009-06-30
影响因子：
11.1
作者：
Gao, Guanjun;Bi, Xiaolin;Rong, Yikang S.
通讯作者：
Rong, Yikang S.
影响因子：
3.3
作者：
M. Gans;C. Audit;M. Masson
通讯作者：
M. Gans;C. Audit;M. Masson