Ferrichrome, a fungal-type siderophore, confers high ammonium tolerance to fission yeast.
Ferrichrome, a fungal-type siderophore, confers high ammonium tolerance to fission yeast.
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Ferrichrome是一种真菌型的铁载体，赋予了对裂变酵母的高铵耐受性。
DOI：
10.1038/s41598-022-22108-0
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发表时间：
2022-10-27
影响因子：
4.6
通讯作者：
中科院分区：
文献类型：
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作者：
微生物和植物产生铁载体，其功能是将环境中的铁输送到细胞内，并参与细胞铁的利用和沉积。它们的生物学功能多种多样，尽管人们对它们在初级代谢中的作用知之甚少。铁色素是由非核糖体多肽合成酶(NRPS)合成的真菌类含铁载体。在此，我们证明了铁铬诱导分裂酵母在高铵介质中的适应性生长。铵是一种首选氮源，因为它通过一种称为氮分解代谢抑制(NCR)的机制来抑制不太受欢迎的氮源的吸收和分解代谢，如亮氨酸。因此，在高浓度的铵存在下，具有亮氨酸营养缺陷型的分裂酵母突变细胞的生长受到抑制。这种生长抑制被铁铬以一种依赖于氨基酸转运蛋白Cat1的方式取消。此外，NRPS基因sib1的缺失加剧了过量铵对野生型细胞的生长抑制，该基因负责铁色素的生物合成，这表明内在产生的铁色素在抑制铵的代谢作用中发挥作用。此外，在低糖条件下，铁色素促进了野生型和sib1缺陷细胞的生长。这些结果表明，细胞内的铁调节初级代谢，包括NCR，这是由铁载体介导的。
Microorganisms and plants produce siderophores, which function to transport environmental iron into cells as well as participate in cellular iron use and deposition. Their biological functions are diverse although their role in primary metabolism is poorly understood. Ferrichrome is a fungal-type siderophore synthesized by nonribosomal peptide synthetase (NRPS). Herein we show that ferrichrome induces adaptive growth of fission yeast on high ammonium media. Ammonium is a preferred nitrogen source as it suppresses uptake and catabolism of less preferred nitrogen sources such as leucine through a mechanism called nitrogen catabolite repression (NCR). Therefore, the growth of fission yeast mutant cells with leucine auxotrophy is suppressed in the presence of high concentrations of ammonium. This growth suppression was canceled by ferrichrome in a manner dependent on the amino acid transporter Cat1. Additionally, growth retardation of wild-type cells by excess ammonium was exacerbated by deleting the NRPS gene sib1, which is responsible for the biosynthesis of ferrichrome, suggesting that intrinsically produced ferrichrome functions in suppressing the metabolic action of ammonium. Furthermore, ferrichrome facilitated the growth of both wild-type and sib1-deficient cells under low glucose conditions. These results suggest that intracellular iron regulates primary metabolism, including NCR, which is mediated by siderophores.
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影响因子：
--
作者：
D'Onofrio A;Crawford JM;Stewart EJ;Witt K;Gavrish E;Epstein S;Clardy J;Lewis K
通讯作者：
Lewis K
影响因子：
2.4
作者：
Nakashima A;Kamada S;Tamanoi F;Kikkawa U
通讯作者：
Kikkawa U
影响因子：
4.4
作者：
Mercier, Alexandre;Labbe, Simon
通讯作者：
Labbe, Simon
影响因子：
--
作者：
Petersen, Janni;Russell, Paul
通讯作者：
Russell, Paul
影响因子：
14.9
作者：
Pelletier, B;Beaudoin, J;Labbé, S
通讯作者：
Labbé, S