Characterisation of a stereotypical cellular and extracellular adult liver progenitor cell niche in rodents and diseased human liver.
Characterisation of a stereotypical cellular and extracellular adult liver progenitor cell niche in rodents and diseased human liver.
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DOI：
10.1136/gut.2009.182345
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发表时间：
2010-05
期刊：
影响因子：
24.5
通讯作者：
Forbes SJ
中科院分区：
文献类型：
--
作者：
Lorenzini S;Bird TG;Boulter L;Bellamy C;Samuel K;Aucott R;Clayton E;Andreone P;Bernardi M;Golding M;Alison MR;Iredale JP;Forbes SJ
组织中的干/祖细胞生态位通过局部信号调节干/祖细胞的分化和增殖。目的：研究干细胞前体细胞的组成和形成。研究了啮齿类动物和人肝损伤和肝祖细胞激活的独立模型中肝祖细胞生态位的组成。为了确定祖细胞和壁龛细胞的来源，采用了性别不匹配的小鼠骨髓移植，这些小鼠接受了胆碱-乙硫氨酸缺乏的饮食诱导的肝损伤和祖细胞的激活。通过免疫组织化学染色描述祖细胞周围的基质，并在使用不同基质层的细胞培养实验中研究其对祖细胞行为的控制作用。肝损伤中的祖细胞反应被肌成纤维细胞和巨噬细胞紧密包围，在较小程度上被内皮细胞包围。肝祖细胞不是骨髓源性的，但骨髓源性细胞与这些细胞密切相关，是巨噬细胞。层粘连蛋白总是围绕在祖细胞周围。体外研究表明，层粘连蛋白有助于维持祖细胞和胆管细胞的表型，促进它们的基因表达(Dlk1、Aquaporin 1、γGT)，同时抑制肝细胞的分化和基因表达(CEPB/α)。在啮齿类动物和人类的肝脏损伤过程中，肝祖细胞周围形成了一个典型的细胞和层粘连蛋白壁龛。层粘连蛋白有助于维持未分化的祖细胞。这个壁龛将肝脏内的祖细胞与骨髓来源的细胞联系起来，并将组织损伤与祖细胞介导的组织修复联系起来。
Stem/progenitor cell niches in tissues regulate stem/progenitor cell differentiation and proliferation through local signalling. To examine the composition and formation of stem progenitor cell niches. The composition of the hepatic progenitor cell niche in independent models of liver injury and hepatic progenitor cell activation in rodents and humans was studied. To identify the origin of the progenitor and niche cells, sex-mismatched bone marrow transplants in mice, who had received the choline—ethionine-deficient-diet to induce liver injury and progenitor cell activation, were used. The matrix surrounding the progenitor cells was described by immunohistochemical staining and its functional role controlling progenitor cell behaviour was studied in cell culture experiments using different matrix layers. The progenitor cell response in liver injury is intimately surrounded by myofibroblasts and macrophages, and to a lesser extent by endothelial cells. Hepatic progenitor cells are not of bone marrow origin; however, bone marrow-derived cells associate intimately with these cells and are macrophages. Laminin always surrounds the progenitor cells. In vitro studies showed that laminin aids maintenance of progenitor and biliary cell phenotype and promotes their gene expression (Dlk1, Aquaporin 1, γGT) while inhibiting hepatocyte differentiation and gene expression (CEPB/α). During liver damage in rodents and humans a stereotypical cellular and laminin niche forms around hepatic progenitor cells. Laminin helps maintenance of undifferentiated progenitor cells. The niche links the intrahepatic progenitor cells with bone marrow-derived cells and links tissue damage with progenitor cell-mediated tissue repair.
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影响因子：
29.4
作者：
Oh, Seh-Hoon;Witek, Rafal P.;Petersen, Bryon E.
通讯作者：
Petersen, Bryon E.
影响因子：
15.9
作者：
Duffield, JS;Forbes, SJ;Iredale, JP
通讯作者：
Iredale, JP
影响因子：
56.9
作者：
Xie, T;Spradling, AC
通讯作者：
Spradling, AC
影响因子：
25.7
作者：
Alison, M;Golding, M;Sarraf, C
通讯作者：
Sarraf, C
DOI：
10.1016/j.bbrc.2009.02.079
发表时间：
2009-04-17
影响因子：
3.1
作者：
Clayton, Elizabeth;Forbes, Stuart J.
通讯作者：
Forbes, Stuart J.