Identification of microRNAs expressed in two mosquito vectors, Aedes albopictus and Culex quinquefasciatus.
Identification of microRNAs expressed in two mosquito vectors, Aedes albopictus and Culex quinquefasciatus.
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DOI：
10.1186/1471-2164-11-119
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发表时间：
2010-02-18
期刊：
影响因子：
4.4
通讯作者：
Cullen BR
中科院分区：
文献类型：
--
作者：
Skalsky RL;Vanlandingham DL;Scholle F;Higgs S;Cullen BR
MicroRNAs （miRNAs）是一种小的非编码rna，在多种生物（包括昆虫、脊椎动物和植物）中转录后调节基因表达。mirna在细胞发育和分化以及细胞对应激和感染的反应中发挥重要作用。迄今为止，在蚊子中鉴定miRNA的报道有限，蚊子是许多人类病原体（包括黄病毒）传播的重要媒介。西尼罗河病毒（WNV）和登革热病毒属黄病毒科，主要由伊蚊和库蚊传播。利用高通量深度测序技术，我们检测了伊蚊的miRNA库。白纹伊蚊细胞和Cx。quinquefasciatus蚊子。我们在Ae中共鉴定了65个mirna。白纹伊蚊C7/10细胞株和77个mirna。致倦库蚊，其中大部分保存在其他昆虫中，如黑腹果蝇和冈比亚按蚊。在两种蚊子中表达最高的miRNA是miR-184，这是一种从昆虫到脊椎动物都保守的miRNA。先前报道的几种按蚊mirna，包括miR-1890和miR-1891，也在库蚊和伊蚊中发现，并且似乎仅限于蚊子。我们在C7/10细胞和Cx中鉴定了7种新的mirna，它们来自9种不同的前体。致倦库蚊，其中两种预测在安省有直系同源。冈比亚按蚊。其中一些新的mirna存在于伊蚊和库蚊中约350 nt长的集群中。引物延伸分析证实miRNA表达。为了确定黄病毒感染是否影响miRNA的表达，我们用西尼罗河病毒感染雌性库蚊。两种mirna miR-92和miR-989在西尼罗河病毒感染后表达水平发生显著变化。伊蚊和库蚊是重要的黄病毒载体。蚊子基因组学和高通量测序技术的最新进展使我们能够询问这两个物种的miRNA谱。在这里，我们提供了超过60个保守的和7个新的蚊子miRNAs的证据，扩展了我们目前对昆虫miRNAs的理解。毫无疑问，鉴定出的一些mirna不仅在蚊子发育过程中发挥作用，而且在蚊子宿主中介导病毒感染。
MicroRNAs (miRNAs) are small non-coding RNAs that post-transcriptionally regulate gene expression in a variety of organisms, including insects, vertebrates, and plants. miRNAs play important roles in cell development and differentiation as well as in the cellular response to stress and infection. To date, there are limited reports of miRNA identification in mosquitoes, insects that act as essential vectors for the transmission of many human pathogens, including flaviviruses. West Nile virus (WNV) and dengue virus, members of the Flaviviridae family, are primarily transmitted by Aedes and Culex mosquitoes. Using high-throughput deep sequencing, we examined the miRNA repertoire in Ae. albopictus cells and Cx. quinquefasciatus mosquitoes. We identified a total of 65 miRNAs in the Ae. albopictus C7/10 cell line and 77 miRNAs in Cx. quinquefasciatus mosquitoes, the majority of which are conserved in other insects such as Drosophila melanogaster and Anopheles gambiae. The most highly expressed miRNA in both mosquito species was miR-184, a miRNA conserved from insects to vertebrates. Several previously reported Anopheles miRNAs, including miR-1890 and miR-1891, were also found in Culex and Aedes, and appear to be restricted to mosquitoes. We identified seven novel miRNAs, arising from nine different precursors, in C7/10 cells and Cx. quinquefasciatus mosquitoes, two of which have predicted orthologs in An. gambiae. Several of these novel miRNAs reside within a ~350 nt long cluster present in both Aedes and Culex. miRNA expression was confirmed by primer extension analysis. To determine whether flavivirus infection affects miRNA expression, we infected female Culex mosquitoes with WNV. Two miRNAs, miR-92 and miR-989, showed significant changes in expression levels following WNV infection. Aedes and Culex mosquitoes are important flavivirus vectors. Recent advances in both mosquito genomics and high-throughput sequencing technologies enabled us to interrogate the miRNA profile in these two species. Here, we provide evidence for over 60 conserved and seven novel mosquito miRNAs, expanding upon our current understanding of insect miRNAs. Undoubtedly, some of the miRNAs identified will have roles not only in mosquito development, but also in mediating viral infection in the mosquito host.
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影响因子：
56.9
作者：
Lagos-Quintana, M;Rauhut, R;Tuschl, T
通讯作者：
Tuschl, T
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Bartel, DP
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Rajewsky, N