Quantifying T lymphocyte turnover.
Quantifying T lymphocyte turnover.
复制标题
DOI：
10.1016/j.jtbi.2012.12.025
复制
发表时间：
2013-06-21
影响因子：
2
通讯作者：
Perelson, Alan S.
中科院分区：
文献类型：
--
作者：
De Boer, Rob J.;Perelson, Alan S.
外周 T 细胞群通过胸腺中幼稚 T 细胞的产生、活化细胞的克隆扩增、细胞自我更新（或稳态增殖）和密度依赖性细胞寿命来维持。已采用各种实验技术来量化这些过程的相对贡献。在现代研究中，淋巴细胞通常用 5-溴-2′-脱氧尿苷 (BrdU)、氘或荧光染料羧基荧光素二乙酸琥珀酰亚胺酯 (CFSE) 进行标记，通过监测端粒缩短和 T 细胞受体切除环 (TREC) 或染料 CFSE 的稀释来研究淋巴细胞的分裂历史，并通过记录抗原特异性细胞群体密度的变化来记录克隆扩增。事实证明，根据 T 细胞产生、分裂和死亡的基本速率正确解释此类数据非常困难，并且涉及数学建模。我们回顾了为每种技术开发的各种模型，讨论哪些模型最适合哪种类型的数据，揭示需要更好模型的开放问题，并查明数学模型背后的假设如何影响数据的解释。这篇综述详细阐述了建模为 T 细胞群动态提供新见解的各种成功案例，对小鼠和男性中涉及维持幼稚和记忆、CD4+ 和 CD8+ T 细胞池的几个过程进行了定量估计。
Peripheral T cell populations are maintained by production of naive T cells in the thymus, clonal expansion of activated cells, cellular self-renewal (or homeostatic proliferation), and density dependent cell life spans. A variety of experimental techniques have been employed to quantify the relative contributions of these processes. In modern studies lymphocytes are typically labeled with 5-bromo-2′-deoxyuridine (BrdU), deuterium, or the fluorescent dye carboxy-fluorescein diacetate succinimidyl ester (CFSE), their division history has been studied by monitoring telomere shortening and the dilution of T cell receptor excision circles (TRECs) or the dye CFSE, and clonal expansion has been documented by recording changes in the population densities of antigen specific cells. Proper interpretation of such data in terms of the underlying rates of T cell production, division, and death has proven to be notoriously difficult and involves mathematical modeling. We review the various models that have been developed for each of these techniques, discuss which models seem most appropriate for what type of data, reveal open problems that require better models, and pinpoint how the assumptions underlying a mathematical model may influence the interpretation of data. Elaborating various successful cases where modeling has delivered new insights in T cell population dynamics, this review provides quantitative estimates of several processes involved in the maintenance of naive and memory, CD4+ and CD8+ T cell pools in mice and men.
登录
查看更多内容
胸腺和最近的胸腺移民在维持成年外周淋巴细胞库中的作用。
影响因子：
15.3
作者：
Berzins, S P;Boyd, R L;Miller, J F
通讯作者：
Miller, J F
DOI：
10.1073/pnas.1019706108
发表时间：
2011-02-22
影响因子：
11.1
作者：
Bocharov, Gennady;Quiel, Juan;Grossman, Zvi
通讯作者：
Grossman, Zvi
DOI：
10.1084/jem.20001021
发表时间：
2002-03-04
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Blattman JN;Antia R;Sourdive DJ;Wang X;Kaech SM;Murali-Krishna K;Altman JD;Ahmed R
通讯作者：
Ahmed R
影响因子：
2.2
作者：
Banks, H. T.;Sutton, Karyn L.;Thompson, W. Clayton;Bocharov, Gennady;Doumic, Marie;Schenkel, Tim;Argilaguet, Jordi;Giest, Sandra;Peligero, Cristina;Meyerhans, Andreas
通讯作者：
Meyerhans, Andreas
DOI：
10.1073/pnas.96.17.9787
发表时间：
1999-08-17
影响因子：
11.1
作者：
Berzins, SP;Godfrey, DI;Boyd, RL
通讯作者：
Boyd, RL