Characterization and Expression Pattern of a Putative Pectin Methylesterase Gene, BcMF27, in Brassica campestris ssp. chinensis
Characterization and Expression Pattern of a Putative Pectin Methylesterase Gene, BcMF27, in Brassica campestris ssp. chinensis
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甘蓝中假定的果胶甲酯酶基因 BcMF27 的特征和表达模式。
DOI：
10.1134/s1021443718030159
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发表时间：
2018-05
影响因子：
1.4
通讯作者：
曹家树
中科院分区：
文献类型：
--
作者：
岳晓艳;曹家树
果胶甲酯酶（PMEs）在细胞壁修饰中起重要作用。PME催化果胶的脱酯化作用，从而影响植物繁殖中的育性。尽管果胶甲酯酶在花卉植物生殖发育中起着重要作用，但近年来对其分子机理的研究却很少。本文对从白菜中分离的BcMF 27（Brassica campestris Male Fertility 27）（布拉德：Bra 000541 GenBank：KT 600012）序列进行了生物信息学分析。SSP. chinensis的果胶甲酯酶结构高度保守。在洋葱表皮细胞中的瞬时表达分析表明，BcMF 27的产物是一个跨膜蛋白。实时荧光定量RT-PCR和原位杂交结果表明，BcMF 27在花粉粒和花粉管中均有表达。本研究表明BcMF 27编码一个跨膜的花粉和花粉管特异性PME基因，为进一步了解果胶甲酯酶的生物学功能和花粉发育、花粉管生长的分子机制提供了基因工具。
Pectin methylesterases (PMEs) play an important role in modifying cell wall. PMEs catalyze the de-esterification of pectin, an important compound of cell wall, to affect fertility in plant reproduction. However, little especially molecular mechanism about pectin methylesterase is studied in recent years despite its importance to reproductive development in flower plant. Here the bioinformatics analysis of BcMF27 (Brassica campestris Male Fertility 27) (BRAD: Bra000541 GenBank: KT600012) sequence isolated from Brassica campestris L. ssp. chinensis showed its highly and characteristically conserved structure as a pectin methylesterase. Transient expression analysis in the onion epidermal cells revealed the product of BcMF27 was a transmembrane protein. Real-time RT-PCR and in situ hybridization suggested that BcMF27 was expressed in pollen grain and pollen tube. This study demonstrates that BcMF27 encodes a transmembrane pollen- and pollen tube-specific PME gene, and is also considered to help further understand the biological function of pectin methylesterases and the molecular mechanism of pollen development, pollen tube growth as a genic tool.
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影响因子：
4.3
作者：
Lionetti, Vincenzo;Cervone, Felice;Bellincampi, Daniela
通讯作者：
Bellincampi, Daniela
影响因子：
11.6
作者：
Di Matteo, A;Giovane, A;Tsernoglou, D
通讯作者：
Tsernoglou, D
DOI：
10.1111/j.1365-313x.2010.04356.x
发表时间：
2010-11
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
A. Yoneda;Takuya Ito;T. Higaki;N. Kutsuna;Tamio Saito;T. Ishimizu;H. Osada;S. Hasezawa;M. Matsui
通讯作者：
A. Yoneda;Takuya Ito;T. Higaki;N. Kutsuna;Tamio Saito;T. Ishimizu;H. Osada;S. Hasezawa;M. Matsui
影响因子：
3.1
作者：
Markovic, O;Janecek, S
通讯作者：
Janecek, S
影响因子：
3.5
作者：
Kanneganti, Vydehi;Gupta, Aditya Kumar
通讯作者：
Gupta, Aditya Kumar