Wheat protein recognition pattern in tolerant and allergic children
Wheat protein recognition pattern in tolerant and allergic children
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耐受和过敏儿童的小麦蛋白识别模式
DOI：
10.1111/pai.12502
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发表时间：
2016
影响因子：
4.4
通讯作者：
Beyer K.
中科院分区：
文献类型：
--
作者：
Sievers S;Rawel H.M;Ringel K.P;Niggemann B;Beyer K.
背景小麦是儿童早期最常见的食物过敏原之一。与其他食物过敏相反，小麦特异性IgE与临床症状严重相关，并且已确定主要用于小麦依赖性运动诱导的过敏反应的相关成分。此外，很高比例的患者存在速发型症状，但小麦特异性IgE不能用市售系统检测。我们解决了小麦过敏（WA）和临床耐受（WT）儿童之间的IgE识别模式是否不同的问题，以确定可用于组分分辨诊断的单个蛋白质。其中44名儿童患有临床相关的小麦过敏，62名儿童对口服食物激发耐受，使用ImmunoCap系统以及针对水和盐溶性以及水不溶性蛋白质组分的免疫印迹分析小麦特异性IgE。40个随机选择的血清进行了分析特异性IgE ω5-gliadin.Results63%的WT和86%的WA儿童致敏小麦与>0.35 kUA/l的免疫CAP分析。我们可以确认α-、β-、γ-和ω-麦醇溶蛋白以及LMW麦谷蛋白亚基作为主要过敏原的作用，并发现IgE与广谱水溶性和盐溶性蛋白条带结合。重要的是，小麦过敏和耐受患者显示IgE结合相同的蛋白条带。WT和WA在ω5-麦醇溶蛋白特异性IgE.Conclusions & Clinical Relevance水平上没有显著差异挑战证明临床相关食物过敏和耐受性食物过敏的儿童具有与相同蛋白条带结合的相似IgE谱。这些发现意味着组分解析诊断可能对小麦过敏的诊断工作没有帮助。
BackgroundWheat is one of the most common food allergens in early childhood. In contrast to other food allergies, wheat‐specific IgE correlates badly with clinical symptoms and relevant components have been identified mostly for wheat‐depended exercise‐induced anaphylaxis. Moreover, a high percentage of patients present with immediate type symptoms but wheat‐specific IgE cannot be detected with commercial available systems.ObjectiveWe addressed the question whether the IgE recognition pattern between wheat allergic (WA) and clinically tolerant (WT) children differs in order to identify individual proteins useful for component‐resolved diagnostics.MethodsSera of 106 children with suspected wheat allergy, of whom 44 children had clinical relevant wheat allergy and 62 were tolerant upon oral food challenge, were analyzed for wheat‐specific IgE using the ImmunoCap system as well as immunoblots against water and salt soluble, and water‐insoluble protein fractions. 40 randomly selected sera were analyzed for specific IgE to ω5‐gliadin.ResultsSixty‐three percent of the WT and 86% of the WA children were sensitized to wheat with >0.35 kUA/l in ImmunoCAP analysis. We could confirm the role of α‐, ß‐, γ‐, and ω‐gliadins, and LMW glutenin subunits as major allergens and found also IgE binding to a broad spectrum of water‐ and salt‐soluble protein bands. It is of great importance that wheat allergic and tolerant patients showed IgE binding to the same protein bands. WT and WA did not significantly differ in levels of ω5‐gliadin‐specific IgE.Conclusions & Clinical RelevanceChildren with challenge proven clinical relevant food allergy and tolerant ones had a similar spectrum of IgE binding to the same protein bands. These findings imply that component‐resolved diagnostics might not be helpful in the diagnostic work‐up of wheat allergy.
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影响因子：
12.4
作者：
Denery-Papini, S.;Bodinier, M.;Moneret-Vautrin, D. -A.
通讯作者：
Moneret-Vautrin, D. -A.
摄入或吸入谷物后的过敏涉及不同年龄段的相似过敏原
影响因子：
6.1
作者：
A. Armentia;R. Rodríguez;A. Callejo;M. Martín‐Esteban;J. Martín‐Santos;G. Salcedo;C. Pascual;R. Sánchez;M. Pardo
通讯作者：
M. Pardo
小麦种子成熟过程中和不同小麦品种中与 IgE 和 IgA 反应的抗原的检测
影响因子：
2.8
作者：
C. Constantin;A. Touraev;E. Heberle‐Bors;S. Quirce;W. Huber;R. Valenta
通讯作者：
R. Valenta
小麦 omega-5 麦醇溶蛋白 IgE 抗体作为儿童小麦过敏诊断工具的作用。
DOI：
10.1016/j.jaci.2008.06.006
发表时间：
2008
期刊：
The Journal of allergy and clinical immunology
影响因子：
--
作者：
K. Beyer;D. Chung;G. Schulz;M. Mishoe;B. Niggemann;U. Wahn;H. Sampson
通讯作者：
H. Sampson
影响因子：
6.8
作者：
Morita, Eishin;Matsuo, Hiroaki;Tanaka, Akira
通讯作者：
Tanaka, Akira