Muscle lineage cytoplasm can change the developmental expression in epidermal lineage cells of ascidian embryos.
Muscle lineage cytoplasm can change the developmental expression in epidermal lineage cells of ascidian embryos.
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肌肉谱系细胞质可以改变海鞘胚胎表皮谱系细胞的发育表达。
DOI：
10.1016/0012-1606(82)90134-8
复制
发表时间：
1982
影响因子：
2.7
通讯作者：
Whittaker,JR
中科院分区：
文献类型：
--
作者：
Whittaker,JR
海鞘胚胎肌肉谱系卵裂球的细胞质可导致非肌肉谱系细胞产生幼虫尾部肌肉乙酰胆碱酯酶。在八细胞阶段，通过显微手术将肌肉细胞质划分为表皮谱系卵裂球。首先通过在四细胞阶段分离前卵裂球对和后卵裂球对获得黑海鞘的后半胚胎（两个B3细胞）。在第三次卵裂时，两个 B3 细胞分裂成仅产生表皮组织的外胚层细胞对和从中衍生出尾肌细胞的中胚层-内胚层卵裂球对。当通过显微外科手术将外胚层和中内胚层卵裂球对彼此分离并作为部分胚胎饲养时，只有源自中内胚层卵裂球的细胞产生组织化学乙酰胆碱酯酶反应。将分离的B3细胞分裂成外胚层和中内胚层细胞对后，立即用微针用力按压中内胚层细胞，使分裂皱纹消失。用微针在中内胚层细胞的新位置重复上下压力，导致在新位置重新形成皱纹，从而合并中胚层细胞质并增加外胚层细胞的大小。通过这种显微外科手术，细胞质改变的外胚层卵裂球对与中内胚层细胞分离，继续分裂并饲养到“幼虫”阶段。这些表皮部分幼虫中有三分之一产生含有乙酰胆碱酯酶的细胞斑。这些结果进一步支持了这样的理论：海鞘胚胎中特定分化途径（胚胎决定）的选择是由特定卵细胞质决定簇的分离介导的。
Cytoplasm from muscle lineage blastomeres of an ascidian embryo can cause cells of a nonmuscle lineage to produce larval tail muscle acetylcholinesterase. Muscle cytoplasm was partitioned microsurgically into epidermal lineage blastomeres at the eight-cell stage. Posterior half-embryos (the two B3 cells) ofAscidia nigrawere obtained first by separating the anterior and posterior blastomere pairs at the four-cell stage. At third cleavage, the two B3 cells divide into an ectodermal cell pair that gives rise solely to epidermal tissues, and a mesodermal-endodermal blastomere pair from which the tail muscle cells are derived. When the ectodermal and mesendodermal blastomere pairs were isolated from one another by microsurgery and reared as partial embryos, only cells originating from the mesendodermal blastomeres produced a histochemical acetylcholinesterase reaction. Immediately after cleavage of the isolated B3 cells into ectodermal and mesendodermal cell pairs, the cleavage furrows could be made to disappear by pressing firmly on the mesendodermal cells with a microneedle. Repeated up and down pressure with the microneedle at a new position across the mesendodermal cells caused furrows to reestablish in the new position, thereby incorporating mesodermal cytoplasm and increasing the size of the ectodermal cells. The cytoplasmically altered ectodermal blastomere pairs, which became detached from the mesendodermal cells by this microsurgical procedure, continued to divide and were reared to “larval” stages. One-third of these epidermal partial larvae produced patches of cells containing acetylcholinesterase. These results lend further support to the theory that choice of particular differentiation pathways (embryonic determination) in ascidian embryos is mediated by segregation of specific egg cytoplasmic determinants.
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将植物极细胞质注射到爪蟾卵中诱导多余的原始生殖细胞
DOI：
--
发表时间：
1978
期刊：
影响因子：
--
作者：
M. Wakahara
通讯作者：
M. Wakahara
DOI：
--
发表时间：
1952
期刊：
影响因子：
--
作者：
P. Kott
通讯作者：
P. Kott
海鞘胚胎发生过程中乙酰胆碱酯酶的发育。
DOI：
--
发表时间：
1979
期刊：
The Journal of experimental zoology
影响因子：
--
作者：
T. H. Meedel;J. Whittaker
通讯作者：
J. Whittaker
DOI：
--
发表时间：
1979
期刊：
影响因子：
--
作者：
J. Whittaker
通讯作者：
J. Whittaker
DOI：
--
发表时间：
1945
期刊：
--
影响因子：
--
作者：
Van Name;G. Willard
通讯作者：
Van Name;G. Willard