Paired immunoglobulin-like receptor B is an entry receptor for mammalian orthoreovirus.
Paired immunoglobulin-like receptor B is an entry receptor for mammalian orthoreovirus.
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DOI：
10.1038/s41467-023-38327-6
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发表时间：
2023-05-05
影响因子：
16.6
通讯作者：
Dermody, Terence S.
中科院分区：
文献类型：
--
作者：
Shang, Pengcheng;Simpson, Joshua D.;Taylor, Gwen M.;Sutherland, Danica M.;Welsh, Olivia L.;Aravamudhan, Pavithra;Natividade, Rita Dos Santos;Schwab, Kristina;Michel, Joshua J.;Poholek, Amanda C.;Wu, Yijen;Rajasundaram, Dhivyaa;Koehler, Melanie;Alsteens, David;Dermody, Terence S.
哺乳动物正呼肠孤病毒（呼肠孤病毒）感染大多数哺乳动物，并与人类的乳糜泻有关。在小鼠中，呼肠孤病毒感染肠道并全身传播，在大脑中引起特定类型的疾病模式。为了鉴定赋予呼肠孤病毒亚型依赖性神经发病机制的受体，我们进行了全基因组CRISPRa筛选，并鉴定了配对的免疫球蛋白样受体B（PirB）作为受体候选物。PirB的异位表达允许呼肠孤病毒结合和感染。PirB细胞外D3D4区是呼肠孤病毒附着和感染性所需的。呼肠孤病毒以通过单分子力光谱法测定的nM亲和力结合PirB。有效的呼肠孤病毒内吞作用需要PirB信号传导基序。在接种的小鼠中，PirB是脑中最大复制和嗜神经血清型3（T3）呼肠孤病毒的完全神经致病性所必需的。在初级皮质神经元中，PirB表达有助于T3呼肠孤病毒感染性。因此，PirB是呼肠孤病毒的进入受体，并有助于T3呼肠孤病毒在鼠脑中的复制和发病。哺乳动物正呼肠孤病毒导致脑中的特定型疾病。在此，Shang等鉴定并证实了成对免疫球蛋白样受体B在呼肠孤病毒附着、进入和感染脑中的作用。
Mammalian orthoreovirus (reovirus) infects most mammals and is associated with celiac disease in humans. In mice, reovirus infects the intestine and disseminates systemically to cause serotype-specific patterns of disease in the brain. To identify receptors conferring reovirus serotype-dependent neuropathogenesis, we conducted a genome-wide CRISPRa screen and identified paired immunoglobulin-like receptor B (PirB) as a receptor candidate. Ectopic expression of PirB allowed reovirus binding and infection. PirB extracelluar D3D4 region is required for reovirus attachment and infectivity. Reovirus binds to PirB with nM affinity as determined by single molecule force spectroscopy. Efficient reovirus endocytosis requires PirB signaling motifs. In inoculated mice, PirB is required for maximal replication in the brain and full neuropathogenicity of neurotropic serotype 3 (T3) reovirus. In primary cortical neurons, PirB expression contributes to T3 reovirus infectivity. Thus, PirB is an entry receptor for reovirus and contributes to T3 reovirus replication and pathogenesis in the murine brain. Mammalian orthoreovirus causes serotype-specific disease in the brain. Here, Shang et al. identify and characterise the role of paired immunoglobulin-like receptor B in reovirus attachment, entry and infectivity in the brain.
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影响因子：
3.4
作者：
Evans, E;Ritchie, K
通讯作者：
Ritchie, K
影响因子：
17.1
作者：
Bochner DN;Sapp RW;Adelson JD;Zhang S;Lee H;Djurisic M;Syken J;Dan Y;Shatz CJ
通讯作者：
Shatz CJ
影响因子：
64.5
作者：
Barton, ES;Forrest, JC;Dermody, TS
通讯作者：
Dermody, TS
DOI：
10.1126/science.aah5298
发表时间：
2017-04-07
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Bouziat R;Hinterleitner R;Brown JJ;Stencel-Baerenwald JE;Ikizler M;Mayassi T;Meisel M;Kim SM;Discepolo V;Pruijssers AJ;Ernest JD;Iskarpatyoti JA;Costes LM;Lawrence I;Palanski BA;Varma M;Zurenski MA;Khomandiak S;McAllister N;Aravamudhan P;Boehme KW;Hu F;Samsom JN;Reinecker HC;Kupfer SS;Guandalini S;Semrad CE;Abadie V;Khosla C;Barreiro LB;Xavier RJ;Ng A;Dermody TS;Jabri B
通讯作者：
Jabri B
影响因子：
56.9
作者：
BELL, GI
通讯作者：
BELL, GI