Novel expression patterns of metabotropic glutamate receptor 6 in the zebrafish nervous system.
Novel expression patterns of metabotropic glutamate receptor 6 in the zebrafish nervous system.
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DOI：
10.1371/journal.pone.0035256
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Neuhauss SC
中科院分区：
文献类型：
--
作者：
Huang YY;Haug MF;Gesemann M;Neuhauss SC
代谢型谷氨酸受体6（mGluR 6或GRM 6）属于代谢型谷氨酸受体家族的III类。它是唯一已知的在神经系统中介导直接突触传递的mGluR，并且被认为介导脊椎动物视网膜的ON途径中的ON反应。系统发育和基因结构分析表明，斑马鱼基因组中含有两个mglur 6旁系同源物，mglur 6a和mglur 6 b。除了在脑的内核层和不同区域中表达外，两种mglur 6旁系同源物在视网膜的神经节细胞中表达，这是一种也可以在下游效应分子gnaoa和gnaob中观察到的表达模式。这种意想不到的表达模式与使用mGluR 6 b特异性肽抗体的免疫组织学标记一致。这些表达模式与现有的观点相矛盾，即mGluR 6仅位于ON-双极细胞上，在那里它在信号传递中起作用。与ON双极细胞中的表达一致，我们报告了基于吗啉的mGluR 6 b下调后视网膜电图中b波振幅降低，显示了ON反应的功能。我们的数据表明mGluR 6介导的信号传导在中枢神经系统中具有更广泛的功能，可能包括内层视网膜中的符号逆转突触。
The metabotropic glutamate receptor 6 (mGluR6 or GRM6) belongs to the class III of the metabotropic glutamate receptor family. It is the only known mGluR that mediates direct synaptic transmission in the nervous system and is thought to mediate the ON-response in the ON-pathway of the vertebrate retina. Phylogenetic and gene structure analysis indicated that the zebrafish genome harbours two mglur6 paralogs, mglur6a and mglur6b. Besides expression in the inner nuclear layer and distinct regions in the brain, both mglur6 paralogs are expressed in ganglion cells of the retina, an expression pattern which can also be observed in the downstream effector molecules gnaoa and gnaob. This unexpected expression pattern is consistent with immunohistological labeling using a peptide antibody specific for the mGluR6b paralog. These expression patterns contradict the existing view that mGluR6 is solely located on ON-bipolar cells where it functions in signal transmission. Consistent with expression in ON-bipolar cells, we report a decreased b-wave amplitude in the electroretinogram after morpholino-based downregulation of mGluR6b, showing a function in the ON response. Our data suggest more widespread functions of mGluR6 mediated signaling in the central nervous system, possibly including sign reversing synapses in the inner retina.
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影响因子：
10.7
作者：
Castresana, J
通讯作者：
Castresana, J
DOI：
10.1073/pnas.0501233102
发表时间：
2005-03-29
影响因子：
11.1
作者：
Dryja, TP;McGee, TL;Rajagopalan, AS
通讯作者：
Rajagopalan, AS
DOI：
10.1016/s0165-3806(97)00066-7
发表时间：
1997-08-18
期刊：
DEVELOPMENTAL BRAIN RESEARCH
影响因子：
--
作者：
Ghosh, PK;Baskaran, N;vandenPol, AN
通讯作者：
vandenPol, AN
影响因子：
3.4
作者：
Gesemann M;Lesslauer A;Maurer CM;Schönthaler HB;Neuhauss SC
通讯作者：
Neuhauss SC
影响因子：
5.3
作者：
Fleisch, Valerie C.;Schonthaler, Helia B.;Neuhauss, Stephan C. F.
通讯作者：
Neuhauss, Stephan C. F.