Combined magnetic resonance and fluorescence imaging of the living mouse brain reveals glioma response to chemotherapy.
Combined magnetic resonance and fluorescence imaging of the living mouse brain reveals glioma response to chemotherapy.
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DOI：
10.1016/j.neuroimage.2008.12.022
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发表时间：
2009-04-01
期刊：
影响因子：
5.7
通讯作者：
Chen JW
中科院分区：
文献类型：
--
作者：
McCann CM;Waterman P;Figueiredo JL;Aikawa E;Weissleder R;Chen JW
荧光分子断层成像（FMT）可以使用特定的荧光探针无创地监测活体动物的分子功能。然而，诸如FMT的宏观成像方法通常表现出低解剖细节。为了克服这一点，我们报告了一种定量技术，图像的结构和功能相结合的FMT和磁共振（MR）成像。我们表明，FMT-MR成像可以产生三维的，多模态图像的活小鼠大脑允许连续监测肿瘤形态和蛋白酶活性。联合FMT-MR肿瘤成像提供了一个独特的体内诊断参数，蛋白酶活性浓度（PAC），它反映了肿瘤的组织学变化，并显着改变全身化疗。该诊断参数的变化在化疗后早期可检测到，并与随后的肿瘤生长相关，预测肿瘤对化疗的反应。我们的研究结果表明，荧光分子探针的组合FMT-MR成像可能对脑肿瘤药物开发和其他神经和躯体成像应用有价值。
Fluorescent molecular tomographic (FMT) imaging can noninvasively monitor molecular function in living animals using specific fluorescent probes. However, macroscopic imaging methods such as FMT generally exhibit low anatomical details. To overcome this, we report a quantitative technique to image both structure and function by combining FMT and magnetic resonance (MR) imaging. We show that FMT-MR imaging can produce three-dimensional, multimodal images of living mouse brains allowing for serial monitoring of tumor morphology and protease activity. Combined FMT-MR tumor imaging provides a unique in vivo diagnostic parameter, protease activity concentration (PAC), which reflects histological changes in tumors and is significantly altered by systemic chemotherapy. Alterations in this diagnostic parameter are detectable early after chemotherapy and correlate with subsequent tumor growth, predicting tumor response to chemotherapy. Our results reveal that combined FMT-MR imaging of fluorescent molecular probes could be valuable for brain tumor drug development and other neurological and somatic imaging applications.
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影响因子：
3.9
作者：
BINDAL, AK;HAMMOUD, M;RAO, JS
通讯作者：
RAO, JS
影响因子：
16.6
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Nesterov, EE;Skoch, J;Swager, TM
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Swager, TM
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Ntziachristos, Vasilis;Bremer, Christoph;Weissleder, Ralph
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Weissleder, Ralph
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Gremlich, HU
影响因子：
3.5
作者：
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通讯作者：
Ntziachristos, V