Comparison of paired human nasal and bronchial airway epithelial cell responses to rhinovirus infection and IL-13 treatment.
Comparison of paired human nasal and bronchial airway epithelial cell responses to rhinovirus infection and IL-13 treatment.
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鼻病毒感染和IL-13治疗对人鼻腔和呼吸道上皮细胞反应的比较。
DOI：
10.1186/s40169-018-0189-2
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发表时间：
2018-05-02
影响因子：
10.6
通讯作者：
Chu HW
中科院分区：
文献类型：
--
作者：
Roberts N;Al Mubarak R;Francisco D;Kraft M;Chu HW
由于其作为微创手术的优势，鼻刷已被越来越多地使用，并被提议作为代替支气管上皮细胞研究下气道疾病的有价值的方法。然而，关于鼻样本是否可以替代支气管样本，存在有限或相互矛盾的证据。本研究的目的是测试鼻上皮细胞是否对IL-13治疗和鼻病毒感染具有相似的抗病毒和炎症反应，鼻病毒感染是一种模拟病毒诱导的哮喘恶化的疾病。在不存在或存在鼻病毒和IL-13处理的情况下，将取自同一患者的鼻和支气管气道上皮细胞在浸没和气液界面（ALI）培养下培养。检测炎性细胞因子IP-10和eotaxin-3、抗病毒基因Mx 1和病毒水平。在没有IL-13处理的情况下，鼻和支气管细胞在ALI和浸没培养物中显示出相似的IP-10应答。在ALI文化下，短期（例如，IL-13处理对两种细胞类型中的病毒和Mx 1水平具有最小的影响。然而，长期（例如，IL-13处理在两种细胞类型中都降低了病毒载量和Mx 1表达。在浸没培养下，两种细胞类型中的IL-13处理对病毒载量、IP-10和Mx 1的影响最小。IL-13诱导的嗜酸性粒细胞趋化因子-3的产生在两种类型的细胞中相似，无论是浸没或ALI培养，这是不受病毒感染。我们的数据表明，鼻上皮细胞可以作为替代支气管上皮细胞在未来的研究，旨在确定2型细胞因子IL-13在调节促炎和抗病毒反应的作用。
Because of its advantage as a minimally invasive procedure, nasal brushings have been increasingly used and proposed as a valuable approach to study lower airway diseases in lieu of bronchial epithelial cells. However, there is limited or conflicting evidence pertaining to whether nasal samples can be surrogates to bronchial samples. The goal of the present study is to test whether nasal epithelial cells have similar antiviral and inflammatory responses to IL-13 treatment and rhinovirus infection, a condition mimicking virally induced asthma exacerbation. Nasal and bronchial airway epithelial cells taken from the same patient were cultured under submerged and air–liquid interface (ALI) culture in the absence or presence of rhinovirus and IL-13 treatment. Inflammatory cytokines IP-10 and eotaxin-3, antiviral gene Mx1 and viral levels were measured. In the absence of IL-13 treatment, nasal and bronchial cells showed a similar IP-10 response in both ALI and submerged cultures. Under the ALI culture, short term (e.g., 3 days) IL-13 treatment had a minimal effect on viral and Mx1 levels in both cell types. However, prolonged (e.g., 14 days) IL-13 treatments in both cell types decreased viral load and Mx1 expression. Under the submerged culture, IL-13 treatment in both cell types has minimal effects on viral load, IP-10 and Mx1. IL-13-induced eotaxin-3 production was similar in both types of cells under either submerged or ALI culture, which was not affected by viral infection. Our data suggest that nasal epithelial cells could serve as a surrogate to bronchial epithelial cells in future studies aimed at defining the role of type 2 cytokine IL-13 in regulating pro-inflammatory and antiviral responses.
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影响因子：
3.7
作者：
Berman R;Jiang D;Wu Q;Stevenson CR;Schaefer NR;Chu HW
通讯作者：
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