Low genetic diversity of Plasmodium falciparum merozoite surface protein 1 and 2 and multiplicity of infections in western Ethiopia following effective malaria interventions.
Low genetic diversity of Plasmodium falciparum merozoite surface protein 1 and 2 and multiplicity of infections in western Ethiopia following effective malaria interventions.
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DOI：
10.1186/s12936-022-04394-1
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发表时间：
2022-12-15
期刊：
影响因子：
3
通讯作者：
Golassa, Lemu
中科院分区：
文献类型：
--
作者：
Tadele, Geletta;Jaiteh, Fatou K.;Oboh, Mary;Oriero, Eniyou;Dugassa, Sisay;Amambua-Ngwa, Alfred;Golassa, Lemu
疟疾寄生虫的遗传多样性可以告知传播的强度，并对疟疾控制和消除干预措施构成重大威胁。遗传多样性的表征将为正在进行的控制工作提供重要信息。本研究旨在探讨裂殖子表面蛋白 1 (msp1) 和裂殖子表面蛋白 2 (msp2) 的等位基因多态性，以确定埃塞俄比亚西部高传播点和低传播点的恶性疟原虫感染的遗传多样性和多重性。从埃塞俄比亚西部确诊的无并发症恶性疟原虫疟疾感染患者收集的总共 225 个干血点中提取了寄生虫基因组 DNA。其中，72.4%（163/225）和27.6%（62/225）的样本分别采集于高透射率和低透射率区域。利用多态性 msp1 和 msp2 基因探索恶性疟疾感染的遗传多样性和多重性。从高传播区域和低传播区域采集的样本中，msp1 基因分型成功率分别为 86.5% (141/163) 和 88.7% (55/62)。在高传播地点和低传播地点采集的样本中分别有 85.3% (139/163) 和 96.8% (60/62) 进行了 msp2 基因分型。通过巢式PCR扩增恶性疟原虫msp1和msp2基因，并通过QIAxcel ScreenGel软件分析PCR产物。 P 值小于或等于 0.05 被认为是显着的。与≥15岁儿童相比，15岁以下儿童恶性疟疾患病率较高（AOR = 2.438，P = 0.005）。在高透射率和低透射率区域收集的样本中观察到 msp1 的三个等位基因家族（K1、MAD20 和 RO33）和 msp2 的两个等位基因家族（FC27 和 3D7）。然而，MAD 20 和 FC 27 等位基因是这两种情况下的主要等位基因家族。在埃塞俄比亚西部流行的恶性疟原虫分离株的遗传多样性和平均 MOI 较低。与低透射区域（平均 MOI 1.08）相比，高透射区域（平均 MOI 1.104）之间的平均 MOI 没有差异（p > 0.05）。从高传播位点收集的分离株（He = 0.17）中msp1的预期杂合度略高于从低传播位点收集的分离株（He = 0.12）。然而，msp2 的杂合度在两种设置中没有不同（Pfmsp2：高透射率下为 0.04；pfmsp2：低透射率下为 0.03）。埃塞俄比亚西部临床疟疾病例中的恶性疟原虫具有较低的遗传多样性和感染复数，无论采样地点的传播强度如何。这些可能表明埃塞俄比亚疟疾控制战略的有效性；尽管需要进一步的研究来确定具体的干预策略和其他参数如何驱动该模式。
Genetic diversity of malaria parasites can inform the intensity of transmission and poses a major threat to malaria control and elimination interventions. Characterization of the genetic diversity would provide essential information about the ongoing control efforts. This study aimed to explore allelic polymorphism of merozoite surface protein 1 (msp1) and merozoite surface protein 2 (msp2) to determine the genetic diversity and multiplicity of Plasmodium falciparum infections circulating in high and low transmission sites in western Ethiopia. Parasite genomic DNA was extracted from a total of 225 dried blood spots collected from confirmed uncomplicated P. falciparum malaria-infected patients in western Ethiopia. Of these, 72.4% (163/225) and 27.6% (62/225) of the samples were collected in high and low transmission areas, respectively. Polymorphic msp1 and msp2 genes were used to explore the genetic diversity and multiplicity of falciparum malaria infections. Genotyping of msp1 was successful in 86.5% (141/163) and 88.7% (55/62) samples collected from high and low transmission areas, respectively. Genotyping of msp2 was carried out among 85.3% (139/163) and 96.8% (60/62) of the samples collected in high and low transmission sites, respectively. Plasmodium falciparum msp1 and msp2 genes were amplified by nested PCR and the PCR products were analysed by QIAxcel ScreenGel Software. A P-value of less or equal to 0.05 was considered significant. High prevalence of falciparum malaria was identified in children less than 15 years as compared with those ≥ 15 years old (AOR = 2.438, P = 0.005). The three allelic families of msp1 (K1, MAD20, and RO33) and the two allelic families of msp2 (FC27 and 3D7), were observed in samples collected in high and low transmission areas. However, MAD 20 and FC 27 alleles were the predominant allelic families in both settings. Plasmodium falciparum isolates circulating in western Ethiopia had low genetic diversity and mean MOI. No difference in mean MOI between high transmission sites (mean MOI 1.104) compared with low transmission area (mean MOI 1.08) (p > 0.05). The expected heterozygosity of msp1 was slightly higher in isolates collected from high transmission sites (He = 0.17) than in those isolates from low transmission (He = 0.12). However, the heterozygosity of msp2 was not different in both settings (Pfmsp2: 0.04 in high transmission; pfmsp2: 0.03 in low transmission). Plasmodium falciparum from clinical malaria cases in western Ethiopia has low genetic diversity and multiplicity of infection irrespective of the intensity of transmission at the site of sampling. These may be signaling the effectiveness of malaria control strategies in Ethiopia; although further studies are required to determine how specific intervention strategies and other parameters that drive the pattern.
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影响因子：
3
作者：
File T;Chekol T;Solomon G;Dinka H;Golassa L
通讯作者：
Golassa L
DOI：
10.1098/rspb.2015.3038
发表时间：
2016-03-16
影响因子：
4.7
作者：
Bushman, Mary;Morton, Lindsay;Udhayakumar, Venkatachalam
通讯作者：
Udhayakumar, Venkatachalam
DOI：
10.1073/pnas.0702715104
发表时间：
2007-05-08
影响因子：
11.1
作者：
Annan, Zeinab;Durand, Patrick;Renaud, Francois
通讯作者：
Renaud, Francois
影响因子：
3
作者：
Kateera, Fredrick;Nsobya, Sam L.;van Vugt, Michele
通讯作者：
van Vugt, Michele
影响因子：
3.7
作者：
Khan SN;Ali R;Khan S;Rooman M;Norin S;Zareen S;Ali I;Ayaz S
通讯作者：
Ayaz S