Genetic Diversity of Polymorphic Marker Merozoite Surface Protein 1 (Msp-1) and 2 (Msp-2) Genes of Plasmodium falciparum Isolates From Malaria Endemic Region of Pakistan.
Genetic Diversity of Polymorphic Marker Merozoite Surface Protein 1 (Msp-1) and 2 (Msp-2) Genes of Plasmodium falciparum Isolates From Malaria Endemic Region of Pakistan.
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DOI：
10.3389/fgene.2021.751552
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发表时间：
2021
影响因子：
3.7
通讯作者：
Ayaz S
中科院分区：
文献类型：
--
作者：
Khan SN;Ali R;Khan S;Rooman M;Norin S;Zareen S;Ali I;Ayaz S
背景资料：通过多态性研究了解疟原虫物种的遗传多样性可以帮助设计更有效的疟疾控制策略，如新药配方和疫苗的开发。巴基斯坦是恶性疟原虫的中度地方病，但对这种寄生虫的遗传多样性知之甚少。本研究旨在探讨巴基斯坦开伯尔-普赫图赫瓦省疟疾流行区恶性疟原虫msp-1和msp-2基因的分子多样性。 研究方法：从开伯尔-普赫图赫瓦省的四个地区（德拉伊斯梅尔汗、卡拉克、马尔丹和白沙瓦）共采集了199/723份血液样本，经显微镜检测为恶性疟疾阳性。应用巢式PCR技术对恶性疟原虫临床分离株msp-1基因2区和msp-2基因2区的K1、MAD 20、RO 33、FC27和3D 7/IC等位基因家族进行扩增，检测恶性疟原虫临床分离株的遗传多样性。 结果：在199份镜检阳性标本中，192份标本经PCR检测证实为恶性疟原虫。msp-1和msp-2分别观察到97和95个扩增子。共鉴定出33种基因型，其中17种为msp-1（8种K1、6种MAD 20和3种RO 33），16种为msp-2（9种FC 27和7种3D 7/IC）。msp-1基因K1家族的等位基因频率（44.3%）高于MAD 20（33.0%）和RO 33（23.0%）; msp-2基因FC 27家族的等位基因频率（60.0%）高于3D 7/IC（40.0%）。在msp-1和msp-2中未观察到多克隆感染。MSP-1和MSP-2的期望杂合度分别为0.98和0.97。 结论：结论是恶性疟原虫种群具有高度多态性，并且在巴基斯坦开伯尔-普赫图赫瓦省存在多种msp-1和msp-2等位基因变体。
Background: Understanding the genetic diversity of Plasmodium species through polymorphic studies can assist in designing more effective control strategies of malaria like new drug formulation and development of a vaccine. Pakistan is moderate endemic for Plasmodium falciparum, but little is known about the genetic diversity of this parasite. This study aimed to investigate the molecular diversity of P. falciparum based on msp-1 and msp-2 genes in the malaria-endemic regions of Khyber Pakhtunkhwa, Pakistan. Methods: A total of 199/723 blood samples, tested positive by microscopy for falciparum malaria, were collected from four districts (Dera Ismail Khan, Karak, Mardan, and Peshawar) of Khyber Pakhtunkhwa. Nested PCR amplification technique was employed to target block 2 of msp-1 and the central domain of msp-2 genes, including their respective allelic families K1, MAD20, RO33, FC27, and 3D7/IC, and to detect the extent of genetic diversity of P. falciparum clinical isolates. Results: Among the 199 microscopy-positive P. falciparum samples, a total of 192 were confirmed using PCR. Ninety-seven amplicons were observed for msp-1 and 95 for msp-2. A total of 33 genotypes, 17 for msp-1 (eight K1, six MAD20, and three RO33) and 16 for msp-2 (nine FC27 and seven 3D7/IC), were identified. The specific allelic frequency of the K1 family was higher (44.3%) than that of MAD20 (33.0%) and RO33 (23.0%) for msp-1, while the FC27 allelic family was dominant (60.0%) compared with 3D7/IC (40.0%) for msp-2. No polyclonal infection was observed in msp-1 and msp-2. The expected heterozygosity was 0.98 and 0.97 for msp-1 and msp-2, respectively. Conclusion: It was concluded that the P. falciparum populations are highly polymorphic, and diverse allelic variants of msp-1 and msp-2 are present in Khyber Pakhtunkhwa, Pakistan.
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影响因子：
3
作者：
Khatoon L;Baliraine FN;Bonizzoni M;Malik SA;Yan G
通讯作者：
Yan G
影响因子：
3
作者：
Howard, Natasha;Durrani, Naeem;Rowland, Mark
通讯作者：
Rowland, Mark
影响因子：
2
作者：
A-Elbasit, Ishraga E.;ElGhazali, Gehad;Giha, Hayder A.
通讯作者：
Giha, Hayder A.
影响因子：
3
作者：
Ghanchi NK;Mårtensson A;Ursing J;Jafri S;Bereczky S;Hussain R;Beg MA
通讯作者：
Beg MA
DOI：
10.4269/ajtmh.1996.54.632
发表时间：
1996-06-01
影响因子：
3.3
作者：
Contamin, H;Fandeur, T;MercereauPuijalon, O
通讯作者：
MercereauPuijalon, O