Restriction endonuclease mapping of the DNA of Rous-associated virus O reveals extensive homology in structure and sequence with avian sarcoma virus DNA.
Restriction endonuclease mapping of the DNA of Rous-associated virus O reveals extensive homology in structure and sequence with avian sarcoma virus DNA.
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Rous相关病毒O的DNA的限制性核酸内切酶图谱揭示了其与禽肉瘤病毒DNA在结构和序列上的广泛同源性。
DOI：
10.1016/0042-6822(81)90537-7
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发表时间：
1981
期刊：
影响因子：
3.7
通讯作者：
Varmus,HE
中科院分区：
文献类型：
--
作者：
Shank,PR;Hughes,SH;Varmus,HE
We have prepared a physical map of the DNA of Rous-associated virus O (RAV-O), an endogenous virus released by certain chicken lines, in order to examine the relationship between the genome of this virus and closely related proviruses endogenous to chickens. Nineteen recognition sties for 11 restriction endonucleases have been mapped on the unintegrated linear and circular forms of RAV-O DNA isolated from acutely infected quail cells.PvuI is the only enzyme tested which does not cleave RAV-O DNA. Most of the sites (18 of 19) occur at similar or identical positions in the DNA of avian sarcoma virus (ASV). Significant differences between the maps of corrseponding regions of RAV-O and ASV DNA are observed only with those endonucleases which recognize sites encoded near the 3′ terminus of ASV RNA (PvuI andEcoRI). Both ends of RAV-O linear DNA contain sequences copied from both the 3′ and the 5′ ends of viral RNA. Two species of closed circular DNA were found: one the same size as the terminally redundant linear DNA (5.0 × 106Mrand the other lacking ca. 0.35 kb from an end of linear DNA. Thus the unintegrated forms of RAV-O DNA appear structurally similar to those of ASV DNA[Shank, P. R.,et al.(1978b).Cell15, 1383–1395;Hsu, W.,et al.(1978).J. Virol.28, 810–818]; presumably one copy of a ca. 0.35-kb terminal repeat unit is lost during formation of the smaller circle from linear DNA. The following paper illustrates the utility of the physical map for differentiating between endogenous proviruses which might or might not have sequence identity with the RAV-O genome.
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内源性和外源性禽肿瘤病毒基因组之间的核苷酸序列关系
影响因子：
5.4
作者：
J. Coffin;M. Champion;F. Chabot
通讯作者：
F. Chabot
通过染色体分级进行基因定位：珠蛋白基因位于至少两条染色体上，三个雌激素诱导基因位于三条染色体上。
DOI：
10.1073/pnas.76.3.1348
发表时间：
1979
影响因子：
11.1
作者：
S. Hughes;E. Stubblefield;F. Payvar;J. D. Engel;J. Dodgson;D. Spector;B. Cordell;R. Schimke;H. Varmus
通讯作者：
H. Varmus
与禽白血病肉瘤病毒基因相关的鸡基因的遗传和表达。
影响因子：
--
作者：
Harriet L. Robinson
通讯作者：
Harriet L. Robinson
鸡大染色体含有内源性原病毒，微染色体含有与ASV转化基因相关的序列
影响因子：
64.5
作者：
Thomas Padgett;E. Stubblefield;H. Varmus
通讯作者：
H. Varmus
ev 3 是内源病毒的结构基因座，在 63 系鸡的交配中与 gs chf 表型分离。
影响因子：
3.7
作者：
S. Astrin;L. Crittenden;E. Buss
通讯作者：
E. Buss