Multiplexed electrochemical protein detection and translation to personalized cancer diagnostics.
Multiplexed electrochemical protein detection and translation to personalized cancer diagnostics.
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DOI：
10.1021/ac401058v
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发表时间：
2013-06-04
影响因子：
7.4
通讯作者：
Rusling, James F.
中科院分区：
文献类型：
--
作者：
Rusling, James F.
测量多种蛋白质的诊断面板有望为癌症等疾病的早期检测和治疗提供一种新的、个性化的方法。患者血清中的生物标志物蛋白水平可以提供疾病状态的持续更新记录。电化学检测蛋白质的研究已经产生了非常灵敏的方法。大多数利用elisa样夹心免疫分析法结合纳米技术的各个方面。其中一些超灵敏的方法已经扩展到微流控多路复用蛋白质检测，但需要工程解决方案，以在单个设备中从少量患者样本（如一滴血液或组织裂解液）中测量更多的蛋白质。为了实现临床或护理点（POC）的使用，简单和低成本是必不可少的。在多路微流控免疫分析中，所需的试剂添加和洗涤步骤提出了需要创造性工程的重大问题。一个巨大的挑战是开发一种通用的癌症筛查设备，以一种简单、经济有效的方式准确测量50-100种蛋白质。这将需要创造性的解决方案来简化试剂添加和多路复用。
Measuring diagnostic panels of multiple proteins promises a new, personalized approach to early detection and therapy of diseases like cancer. Levels of biomarker proteins in patient serum can provide a continually updated record of disease status. Research in electrochemical detection of proteins has produced exquisitely sensitive approaches. Most utilize ELISA-like sandwich immunoassays incorporating various aspects of nanotechnology. Several of these ultrasensitive methodologies have been extended to microfluidic multiplexed protein detection, but engineered solutions are needed to measure more proteins in a single device from a small patient sample such as a drop of blood or tissue lysate. To achieve clinical or point-of-care (POC) use, simplicity and low cost are essential. In multiplexed microfluidic immunoassays, required reagent additions and washing steps pose a significant problem calling for creative engineering. A grand challenge is to develop a general cancer screening device to accurately measure 50–100 proteins in a simple, cost effective fashion. This will require creative solutions to simplified reagent addition and multiplexing.
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影响因子：
12.6
作者：
Chikkaveeraiah, Bhaskara V.;Mani, Vigneshwaran;Patel, Vyomesh;Gutkind, J. Silvio;Rusling, James F.
通讯作者：
Rusling, James F.
影响因子：
17.1
作者：
Mani, Vigneshwaran;Chikkaveeraiah, Bhaskara V.;Patel, Vyomesh;Gutkind, J. Silvio;Rusling, James F.
通讯作者：
Rusling, James F.
DOI：
10.3390/s100100428
发表时间：
2010
期刊：
Sensors (Basel, Switzerland)
影响因子：
--
作者：
Choi YE;Kwak JW;Park JW
通讯作者：
Park JW
影响因子：
7.4
作者：
Linder, V;Sia, SK;Whitesides, GM
通讯作者：
Whitesides, GM
影响因子：
7.4
作者：
Malhotra, Ruchika;Patel, Vyomesh;Chikkaveeraiah, Bhaskara V.;Munge, Bernard S.;Cheong, Sok Ching;Zain, Rosnah B.;Abraham, Mannil T.;Dey, Dipak K.;Gutkind, J. Silvio;Rusling, James F.
通讯作者：
Rusling, James F.