Thermal stress accelerates Arabidopsis thaliana mutation rate.
Thermal stress accelerates Arabidopsis thaliana mutation rate.
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DOI：
10.1101/gr.259853.119
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发表时间：
2021-01
期刊：
影响因子：
7
通讯作者：
Harberd NP
中科院分区：
文献类型：
--
作者：
Belfield EJ;Brown C;Ding ZJ;Chapman L;Luo M;Hinde E;van Es SW;Johnson S;Ning Y;Zheng SJ;Mithani A;Harberd NP
突变是遗传多样性和突变负荷的来源。然而，环境温度升高对植物突变率的影响以及对特定突变类别（例如，插入/缺失[indel]相对于单核苷酸变体[SNV]）。这一主题之所以重要，是因为人类活动造成的全球气温上升对生物系统的影响尚未明确界定。在这里，我们展示了温度升高对拟南芥突变的影响，研究了在29°C下连续生长11代的突变积累（MA）谱系的全基因组图谱。而A.在标准温度（ST; 23°C）下生长的拟南芥与每代每个位点7 × 10−9个碱基替换的突变率相关，在胁迫高温（HT; 29°C）下生长的拟南芥具有高度的诱变性，突变率增加到12 × 10−9。在HT时SNV频率比在ST时高大约2 - 3倍，并且HT生长导致插入缺失频率增加19- 23倍，导致插入缺失（相对于SNV）不成比例地增加。大多数HT诱导的插入缺失的大小为1-2 bp，并且特别影响基因组的均聚或二核苷酸A或T拉伸区域。HT诱导的插入缺失不成比例地发生在无核小体的区域，这表明当基因组DNA被包装到核小体中时，在细胞周期阶段发生了许多HT诱导的突变损伤。我们的结论是，压力实验温度的增加加速植物突变率，特别是加速插入缺失突变的速率。因此，环境温度的升高可能对野生植物产生显著的诱变后果，特别是可能在遗传上增加突变负荷。
Mutations are the source of both genetic diversity and mutational load. However, the effects of increasing environmental temperature on plant mutation rates and relative impact on specific mutational classes (e.g., insertion/deletion [indel] vs. single nucleotide variant [SNV]) are unknown. This topic is important because of the poorly defined effects of anthropogenic global temperature rise on biological systems. Here, we show the impact of temperature increase on Arabidopsis thaliana mutation, studying whole genome profiles of mutation accumulation (MA) lineages grown for 11 successive generations at 29°C. Whereas growth of A. thaliana at standard temperature (ST; 23°C) is associated with a mutation rate of 7 × 10−9 base substitutions per site per generation, growth at stressful high temperature (HT; 29°C) is highly mutagenic, increasing the mutation rate to 12 × 10−9. SNV frequency is approximately two- to threefold higher at HT than at ST, and HT-growth causes an ∼19- to 23-fold increase in indel frequency, resulting in a disproportionate increase in indels (vs. SNVs). Most HT-induced indels are 1–2 bp in size and particularly affect homopolymeric or dinucleotide A or T stretch regions of the genome. HT-induced indels occur disproportionately in nucleosome-free regions, suggesting that much HT-induced mutational damage occurs during cell-cycle phases when genomic DNA is packaged into nucleosomes. We conclude that stressful experimental temperature increases accelerate plant mutation rates and particularly accelerate the rate of indel mutation. Increasing environmental temperatures are thus likely to have significant mutagenic consequences for plants growing in the wild and may, in particular, add detrimentally to mutational load.
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DOI：
10.1016/j.cub.2011.07.002
发表时间：
2011-08-23
期刊：
Current biology : CB
影响因子：
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作者：
Jiang C;Mithani A;Gan X;Belfield EJ;Klingler JP;Zhu JK;Ragoussis J;Mott R;Harberd NP
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影响因子：
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作者：
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通讯作者：
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