Environmentally responsive genome-wide accumulation of de novo Arabidopsis thaliana mutations and epimutations.
Environmentally responsive genome-wide accumulation of de novo Arabidopsis thaliana mutations and epimutations.
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从头响应的拟南芥突变和表述的环境响应式全基因组的积累。
DOI：
10.1101/gr.177659.114
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发表时间：
2014-11
期刊：
影响因子：
7
通讯作者：
Harberd NP
中科院分区：
文献类型：
--
作者：
Jiang C;Mithani A;Belfield EJ;Mott R;Hurst LD;Harberd NP
进化是由表型多样性推动的，而表型多样性又是由于潜在的遗传（和潜在的表观遗传）变异。虽然众所周知环境因素会影响微生物和人类癌细胞中新变异的积累，但自然环境影响植物中新变异积累的程度相对未知。在这里，我们使用全基因组和全甲基化组测序测试，如果一个特定的环境压力（高盐土壤）改变的频率和积累的突变和表突变（胞嘧啶甲基化状态的变化）突变积累（MA）的拟南芥谱系的分子概况。我们首先表明，应激谱系积累了100%以上的突变，并且这些突变表现出独特的分子突变谱（颠换和插入/缺失[indel]突变相对频率的特定增加）。我们接下来表明，应激谱系在CG位点（CG-DMPs）处积累的差异甲基化胞嘧啶位置（DMPs）比对照多1.45%，并且还表明，虽然这些CG-DMPs中的许多（1.75%）是遗传的，但有些可能在后代中丢失。最后，我们表明，压力相关CG-DMPs出现更频繁地在基因比非基因区域的基因组。我们认为，通常遇到的自然环境压力可以加速积累和改变植物中的新的遗传变异的配置文件。我们的研究结果是重要的，因为压力暴露在野生植物中很常见，他们表明环境因素可能会显着改变燃料植物进化的遗传性新变体的发病率和模式。
Evolution is fueled by phenotypic diversity, which is in turn due to underlying heritable genetic (and potentially epigenetic) variation. While environmental factors are well known to influence the accumulation of novel variation in microorganisms and human cancer cells, the extent to which the natural environment influences the accumulation of novel variation in plants is relatively unknown. Here we use whole-genome and whole-methylome sequencing to test if a specific environmental stress (high-salinity soil) changes the frequency and molecular profile of accumulated mutations and epimutations (changes in cytosine methylation status) in mutation accumulation (MA) lineages of Arabidopsis thaliana. We first show that stressed lineages accumulate ∼100% more mutations, and that these mutations exhibit a distinctive molecular mutational spectrum (specific increases in relative frequency of transversion and insertion/deletion [indel] mutations). We next show that stressed lineages accumulate ∼45% more differentially methylated cytosine positions (DMPs) at CG sites (CG-DMPs) than controls, and also show that while many (∼75%) of these CG-DMPs are inherited, some can be lost in subsequent generations. Finally, we show that stress-associated CG-DMPs arise more frequently in genic than in nongenic regions of the genome. We suggest that commonly encountered natural environmental stresses can accelerate the accumulation and change the profiles of novel inherited variants in plants. Our findings are significant because stress exposure is common among plants in the wild, and they suggest that environmental factors may significantly alter the rates and patterns of incidence of the inherited novel variants that fuel plant evolution.
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影响因子：
4.5
作者：
Baxter I;Brazelton JN;Yu D;Huang YS;Lahner B;Yakubova E;Li Y;Bergelson J;Borevitz JO;Nordborg M;Vitek O;Salt DE
通讯作者：
Salt DE
影响因子：
56.9
作者：
Jacobsen, SE;Meyerowitz, EM
通讯作者：
Meyerowitz, EM
DOI：
10.1016/j.cub.2011.07.002
发表时间：
2011-08-23
期刊：
Current biology : CB
影响因子：
--
作者：
Jiang C;Mithani A;Gan X;Belfield EJ;Klingler JP;Zhu JK;Ragoussis J;Mott R;Harberd NP
通讯作者：
Harberd NP
影响因子：
64.8
作者：
Gan X;Stegle O;Behr J;Steffen JG;Drewe P;Hildebrand KL;Lyngsoe R;Schultheiss SJ;Osborne EJ;Sreedharan VT;Kahles A;Bohnert R;Jean G;Derwent P;Kersey P;Belfield EJ;Harberd NP;Kemen E;Toomajian C;Kover PX;Clark RM;Rätsch G;Mott R
通讯作者：
Mott R
DOI：
10.1073/pnas.1209329109
发表时间：
2012-08-07
影响因子：
11.1
作者：
Dowen, Robert H.;Pelizzola, Mattia;Ecker, Joseph R.
通讯作者：
Ecker, Joseph R.