Identification of a short nuclear lamin protein selectively expressed during meiotic stages of rat spermatogenesis.
Identification of a short nuclear lamin protein selectively expressed during meiotic stages of rat spermatogenesis.
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鉴定在大鼠精子发生减数分裂阶段选择性表达的短核纤层蛋白。
DOI：
10.1111/j.1432-0436.1992.tb00499.x
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Ricardo Benavente
中科院分区：
文献类型：
--
作者：
Avril Smith;Ricardo Benavente
核层是一种位于核周的核骨架结构，与核内膜紧密相连。它由一个多基因蛋白质家族--Lamins组成，表现出明显的细胞类型特异性表达模式。Lamins的功能作用尚未确定，但现有的信息表明，它们参与了核膜和间期染色质的组织。精子发生的特征之一是染色质组织和功能的阶段性变化。伴随着这些变化的是核层的组织和组成的改变。在以前的实验中，我们已经确定大鼠生精细胞表达的层蛋白与体细胞的层蛋白B1密切相关，如果不是相同的话；而大鼠体细胞层蛋白A、C、D和E没有检测到。考虑到精子发生过程中染色质的重组可能与核层的变化直接或间接相关，我们决定进一步研究这一过程中层蛋白的表达。在这里，我们报告了大鼠52 kDa蛋白的鉴定，根据免疫细胞化学和生化数据，它似乎是一种新的核层蛋白。利用减数分裂阶段的特定标记，我们还证明了这种短层粘连蛋白在精子发生的减数分裂阶段有选择性地表达。
The nuclear lamina is a karyoskeletal structure located at the nuclear periphery and intimately associated with the inner nuclear membrane. It is composed of a multigene family of proteins, the lamins, which show a conspicuous cell type-specific expression pattern. The functional role of lamins has not been definitively established but available information indicates that they are involved in the organization of nuclear envelope and interphase chromatin. Spermatogenesis is characterized, among other features, by stage-specific changes in chromatin organization and function. These changes are accompanied by modifications in the organization and composition of the nuclear lamina. In previous experiments we have determined that rat spermatogenic cells express a lamin closely related, if not identical, to lamin B1 of somatic cells; whereas rat somatic lamins A, C, D and E were not detected. Considering that chromatin reorganizations during spermatogenesis may be directly or indirectly related to changes of the nuclear lamina we have decided to further investigate lamin expression during this process. Here we report on the identification of a 52 kDa protein of the rat which, according to immunocytochemical and biochemical data, appears to be a novel nuclear lamin. Using meiotic stage-specific markers, we have also demonstrated that this short lamin is selectively expressed during meiotic stages of spermatogenesis.
大鼠肝核纤层蛋白多肽家族的其他成员。
DOI：
--
发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Kaufmann,SH
通讯作者：
Kaufmann,SH
核膜中的核纤层蛋白 B 受体。
DOI：
10.1073/pnas.85.22.8531
发表时间：
1988
影响因子：
11.1
作者：
Worman,HJ;Yuan,J;Blobel,G;Georgatos,SD
通讯作者：
Georgatos,SD
影响因子：
3
作者：
North,SimonJ;Koles,Kate;Hembd,Caleb;Morris,HowardR;Dell,Anne;Panin,VladislavM;Haslam,StuartM
通讯作者：
Haslam,StuartM