TERMINAL FLOWER-1/CENTRORADIALIS inhibits tuberisation via protein interaction with the tuberigen activation complex.
TERMINAL FLOWER-1/CENTRORADIALIS inhibits tuberisation via protein interaction with the tuberigen activation complex.
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DOI：
10.1111/tpj.14898
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发表时间：
2020-09
期刊：
影响因子：
--
通讯作者：
Taylor MA
中科院分区：
文献类型：
--
作者：
Zhang X;Campbell R;Ducreux LJM;Morris J;Hedley PE;Mellado-Ortega E;Roberts AG;Stephens J;Bryan GJ;Torrance L;Chapman SN;Prat S;Taylor MA
马铃薯块茎的形成是一种次生发育过程，在此过程中，茎下区域的细胞分裂和径向扩展，进一步分化为积累淀粉的薄壁组织。尽管我们已经知道了块茎形成的分子途径的一些细节，但我们的知识中仍然存在着重要的差距。在这里，端花1/中央辐射基因家族(称为StCEN)的一个成员在块茎形成的负调控中的作用被认为是第一次被证明。结果表明，StCEN基因的低表达加速了块茎的形成，而过度表达该基因的转基因植株则表现出延迟块茎形成和降低块茎产量的现象。蛋白质-蛋白质相互作用研究(酵母双杂交和双分子荧光互补)表明，StCEN与最近描述的结节蛋白激活复合体的组成部分结合。利用瞬时反式激活分析，我们发现StSP6A块茎形成信号是块茎形成激活复合体的激活靶标，并且StCEN的共表达阻止了StFD-like-1对StSP6A基因的激活。错误表达StCEN的转基因株系的转录转录分析确定了块茎形成中的早期转录事件。这些结果表明，StCEN通过直接拮抗StSP6A在匍匐茎中的功能，将StCEN确定为一个育种标记，通过选择StCEN表达降低的基因型来提高块茎分化和产量，从而抑制块茎形成。了解马铃薯块茎形成的机制是提高作物产量的关键途径，而作物的产量对粮食安全至关重要。我们证明了马铃薯磷脂酰乙醇胺结合蛋白基因家族(StCEN)的一个成员是块茎形成调控的关键成分，它通过与块茎形成激活复合体相互作用来调节块茎形成早期的转录变化。
Potato tuber formation is a secondary developmental programme by which cells in the subapical stolon region divide and radially expand to further differentiate into starch‐accumulating parenchyma. Although some details of the molecular pathway that signals tuberisation are known, important gaps in our knowledge persist. Here, the role of a member of the TERMINAL FLOWER 1/CENTRORADIALIS gene family (termed StCEN) in the negative control of tuberisation is demonstrated for what is thought to be the first time. It is shown that reduced expression of StCEN accelerates tuber formation whereas transgenic lines overexpressing this gene display delayed tuberisation and reduced tuber yield. Protein–protein interaction studies (yeast two‐hybrid and bimolecular fluorescence complementation) demonstrate that StCEN binds components of the recently described tuberigen activation complex. Using transient transactivation assays, we show that the StSP6A tuberisation signal is an activation target of the tuberigen activation complex, and that co‐expression of StCEN blocks activation of the StSP6A gene by StFD‐Like‐1. Transcriptomic analysis of transgenic lines misexpressing StCEN identifies early transcriptional events in tuber formation. These results demonstrate that StCEN suppresses tuberisation by directly antagonising the function of StSP6A in stolons, identifying StCEN as a breeding marker to improve tuber initiation and yield through the selection of genotypes with reduced StCEN expression. Understanding the mechanism of tuber initiation in potato provides a key route to increasing productivity of a crop that is essential for food security. We demonstrate that a member of the potato phosphatidylethanolamine‐binding protein gene family (StCEN) is a key component in the regulation of tuberisation that acts via interaction with the tuberigen activation complex to regulate early tuberisation transcriptional changes.
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影响因子：
11.6
作者：
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