Micro anion exchange chromatography of carbohydrate-deficient transferrin in serum in relation to alcohol consumption (Swedish Patent 8400587-5).
Micro anion exchange chromatography of carbohydrate-deficient transferrin in serum in relation to alcohol consumption (Swedish Patent 8400587-5).
复制标题
血清中缺乏碳水化合物的转铁蛋白的微阴离子交换色谱与酒精消耗的关系（瑞典专利 8400587-5）。
DOI：
--
复制
发表时间：
1986
期刊：
影响因子：
--
通讯作者：
M. Joustra
中科院分区：
文献类型：
--
作者：
H. Stibler;S. Borg;M. Joustra
本文介绍了一种简便、快速的检测和定量血清中“缺糖转铁蛋白”的新方法。该方法是基于等度阴离子交换色谱的异转铁蛋白在一次性微柱，然后通过双抗体转铁蛋白放射免疫测定。该技术，分离所有的转铁蛋白组分等电pH值高于5.65，表现出非常好的重现性和准确性与5和9%之间的变异系数。77例酒精中毒患者与80例健康“正常消费者”和33例完全戒酒者的特异性为100%，敏感性为91%。这些值与最近一个月的饮酒量显著相关，并且在戒酒者中下降，平均生物半衰期为17天。在10天内每天摄入60 g乙醇后，健康个体偶尔会出现水平升高。在187例非酒精相关性疾病患者的样本中，仅发现2%的假阳性值，该方法被认为是检测和监测酒精滥用的潜在工具。
A new simplified and rapid method for detection and quantitation of "carbohydrate-deficient transferrin" in serum is described. The method is based on isocratic anion exchange chromatography of isotransferrins in disposable microcolumns followed by a double antibody transferrin radioimmune assay. This technique, which separates all transferrin components isoelectric above pH 5.65, showed a very good reproducibility and accuracy with a coefficient of variation between 5 and 9%. 77 alcoholic patients could be clearly separated from 80 healthy "normal consumers" and 33 total abstainers with a specificity of 100% and a sensitivity of 91%. The values were significantly correlated to the amount of alcohol consumed during the latest month, and declined in abstaining alcoholics with a mean biological half-life of 17 days. Elevated levels occasionally appeared in healthy individuals after daily consumption of 60 g of ethanol during a 10-day period. In a sample of 187 patients with nonalcohol-related conditions only 2% false-positive values were found. This method is suggested as a potential tool for detecting and monitoring alcohol abuse.
在乙醇存在下培养的粘膜细胞合成和分泌胃粘液糖蛋白。
影响因子：
3.9
作者：
Slomiany,A;Takagi,A;Slomiany,BL
通讯作者：
Slomiany,BL
急性乙醇给药后肝脏质膜糖蛋白组装受损。
DOI：
10.1016/s0006-291x(84)80226-0
发表时间：
1984
影响因子：
3.1
作者：
Mailliard,ME;Sorrell,MF;Volentine,GD;Tuma,DJ
通讯作者：
Tuma,DJ