A pilot investigation of visceral fat adiposity and gene expression profile in peripheral blood cells.
A pilot investigation of visceral fat adiposity and gene expression profile in peripheral blood cells.
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DOI：
10.1371/journal.pone.0047377
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Shimomura I
中科院分区：
文献类型：
--
作者：
Yamaoka M;Maeda N;Nakamura S;Kashine S;Nakagawa Y;Hiuge-Shimizu A;Okita K;Imagawa A;Matsuzawa Y;Matsubara K;Funahashi T;Shimomura I
有证据表明，内脏脂肪堆积在代谢综合征的发展中起着核心作用。过多的内脏脂肪导致局部慢性低度炎症和脂肪细胞因子的失调，这在代谢综合征的发病机制中起到了作用。这些变化可能会影响外周血细胞中的基因表达。这项研究首次研究了内脏脂肪肥胖与外周血细胞基因表达谱之间的关系。采用基因芯片技术分析了28例肥胖者外周血细胞的基因表达谱。对57例肥胖者的外周血细胞进行逆转录聚合酶链式反应(RT-PCR)。根据日本标准，肥胖定义为体重指数(BMI)大于25 kg/m2，并通过腹部生物电阻抗测量估计的内脏脂肪面积(EVFA)。采用Agilent全基因组4×44K寡核苷酸芯片进行基因表达谱分析。与昼夜节律、炎症和氧化应激相关的几个基因的表达与内脏脂肪积累显著相关。血细胞周期同源蛋白1(Period Homolog 1，PER1)mRNA水平与内脏脂肪肥胖呈负相关。逐步多元回归分析表明，eVFA是PER1表达的重要决定因素。总而言之，内脏脂肪肥胖与外周血细胞中与昼夜节律和炎症相关的基因表达有关。
Evidence suggests that visceral fat accumulation plays a central role in the development of metabolic syndrome. Excess visceral fat causes local chronic low-grade inflammation and dysregulation of adipocytokines, which contribute in the pathogenesis of the metabolic syndrome. These changes may affect the gene expression in peripheral blood cells. This study for the first time examined the association between visceral fat adiposity and gene expression profile in peripheral blood cells. The gene expression profile was analyzed in peripheral blood cells from 28 obese subjects by microarray analysis. Reverse transcription-polymerase chain reaction (RT-PCR) was performed using peripheral blood cells from 57 obese subjects. Obesity was defined as body mass index (BMI) greater than 25 kg/m2 according to the Japanese criteria, and the estimated visceral fat area (eVFA) was measured by abdominal bioelectrical impedance. Analysis of gene expression profile was carried out with Agilent whole human genome 4×44 K oligo-DNA microarray. The expression of several genes related to circadian rhythm, inflammation, and oxidative stress correlated significantly with visceral fat accumulation. Period homolog 1 (PER1) mRNA level in blood cells correlated negatively with visceral fat adiposity. Stepwise multiple regression analysis identified eVFA as a significant determinant of PER1 expression. In conclusion, visceral fat adiposity correlated with the expression of genes related to circadian rhythm and inflammation in peripheral blood cells.
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昼夜节律的代谢和能量学的融合。
DOI：
10.1126/science.1195027
发表时间：
2010-12-03
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Bass J;Takahashi JS
通讯作者：
Takahashi JS
影响因子：
2.8
作者：
Dallmann R;Weaver DR
通讯作者：
Weaver DR
影响因子：
3.3
作者：
Matsuzawa, Y;Nakamura, T;Nagai, M
通讯作者：
Nagai, M
影响因子：
56.9
作者：
Turek, FW;Joshu, C;Bass, J
通讯作者：
Bass, J
DOI：
10.1016/j.bbrc.2007.01.063
发表时间：
2007-03-23
影响因子：
3.1
作者：
Fukuya, Hiroyuki;Emoto, Noriaki;Yokoyama, Mitsuhiro
通讯作者：
Yokoyama, Mitsuhiro