Convergent targeting of a common host protein-network by pathogen effectors from three kingdoms of life.
Convergent targeting of a common host protein-network by pathogen effectors from three kingdoms of life.
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DOI：
10.1016/j.chom.2014.08.004
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发表时间：
2014-09-10
影响因子：
30.3
通讯作者：
Braun P
中科院分区：
文献类型：
--
作者：
Weßling R;Epple P;Altmann S;He Y;Yang L;Henz SR;McDonald N;Wiley K;Bader KC;Gläßer C;Mukhtar MS;Haigis S;Ghamsari L;Stephens AE;Ecker JR;Vidal M;Jones JD;Mayer KF;Ver Loren van Themaat E;Weigel D;Schulze-Lefert P;Dangl JL;Panstruga R;Braun P
虽然控制植物免疫的概念原理越来越清晰，但其系统级组织和宿主-病原体界面的进化动态仍然模糊不清。我们从子囊菌病原体goovinomyces orontii和拟南芥（Arabidopsis thaliana）宿主蛋白中生成了一个系统的毒力效应蛋白-蛋白相互作用网络。我们将该数据集与真菌性病原体丁香假单胞菌和拟南芥卵菌病原体透明operonospora拟南芥的相应数据相结合。由此产生的网络识别宿主蛋白上的种内和种间病原体效应收敛。124个拟南芥效应相互作用突变体的表型分析揭示了种内和种间趋同与几种改变的免疫反应表型之间的相关性。效应器和最重靶向的宿主蛋白在亚核病灶共定位。自适应选择的拟南芥基因产物丰富，可与效应靶点相互作用。我们的数据表明，在拟南芥中存在一个保守的分子宿主-病原体界面，而进化适应发生在效应靶点的直接网络邻域。
While conceptual principles governing plant immunity are becoming clear, its systems-level organization and the evolutionary dynamic of the host-pathogen interface are still obscure. We generated a systematic protein-protein interaction network of virulence effectors from the ascomycete pathogen Golovinomyces orontii and Arabidopsis thaliana host proteins. We combined this dataset with corresponding data for the eubacterial pathogen Pseudomonas syringae and the oomycete pathogen Hyaloperonospora arabidopsidis. The resulting network identifies host proteins onto which intraspecies and interspecies pathogen effectors converge. Phenotyping of 124 Arabidopsis effector-interactor mutants revealed a correlation between intra- and interspecies convergence and several altered immune response phenotypes. The effectors and most heavily targeted host protein co-localized in sub-nuclear foci. Products of adaptively selected Arabidopsis genes are enriched for interactions with effector targets. Our data suggest the existence of a molecular host-pathogen interface that is conserved across Arabidopsis accessions, while evolutionary adaptation occurs in the immediate network neighborhood of effector targets.
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DOI：
10.1126/science.1236011
发表时间：
2013-08-16
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Dangl JL;Horvath DM;Staskawicz BJ
通讯作者：
Staskawicz BJ
影响因子：
6.7
作者：
Baltrus DA;Nishimura MT;Romanchuk A;Chang JH;Mukhtar MS;Cherkis K;Roach J;Grant SR;Jones CD;Dangl JL
通讯作者：
Dangl JL
影响因子：
30.8
作者：
Consonni, Chiara;Humphry, Matthew E.;Panstruga, Ralph
通讯作者：
Panstruga, Ralph
DOI：
10.1199/tab.0115
发表时间：
2008-01-01
期刊：
The arabidopsis book
影响因子：
--
作者：
Micali, Cristina;Gollner, Katharina;Panstruga, Ralph
通讯作者：
Panstruga, Ralph
影响因子：
9.2
作者：
Chen, M;Tao, Y;Chory, J
通讯作者：
Chory, J