Adhesion of human glioma cell lines to fibronectin, laminin, vitronectin and collagen I is modulated by gangliosides in vitro.
Adhesion of human glioma cell lines to fibronectin, laminin, vitronectin and collagen I is modulated by gangliosides in vitro.
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人神经胶质瘤细胞系对纤连蛋白、层粘连蛋白、玻连蛋白和 I 型胶原蛋白的粘附在体外受到神经节苷脂的调节。
DOI：
10.3109/15419069509081276
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
G. Pilkington
中科院分区：
文献类型：
--
作者：
Abderrahim Merzak;S. Koochekpour;G. Pilkington
研究了8种不同组织学类型的胶质瘤细胞系对纤维连接蛋白、ⅰ型胶原、玻璃体连接蛋白和层粘连蛋白的粘附作用。胶质瘤细胞系对这些基质有不同程度的附着。有趣的是，所有细胞系都与层粘连蛋白紧密相连。此外，胶质瘤细胞黏附是剂量依赖性的。此外，GRGDTP肽部分抑制了三种细胞系对纤维连接蛋白和胶原I的粘附，完全阻止了对玻璃体连接蛋白的粘附，表明整合素受体参与了胶质瘤细胞的粘附。最近，我们已经证明神经节苷类在体外促进神经胶质瘤细胞入侵重建基底膜基质中起重要作用。为了研究神经节苷类在这一过程中的作用机制，我们在三种细胞系中研究了六种神经节苷类（GM1、GM3、GD3、GD1a、GD1b和GT1b）在细胞粘附这四种蛋白中的作用。虽然除GM3外，所有的神经节苷脂都在不同程度上增强了细胞对这些蛋白的粘附，但在所有三种细胞系中，GD3被证明是最有效的促进粘附的神经节苷脂。发现GM3在一个细胞系中抑制细胞对四种蛋白的粘附，但在另外两个细胞系中增强细胞粘附。发现这三种细胞系均表达GD3和A2B5抗体识别的神经节苷脂。此外，三种细胞系对纤维连接蛋白、玻璃体连接蛋白、层粘连蛋白和I型胶原的粘附被A2B5孵育抑制，表明胶质瘤细胞对这些蛋白的粘附参与了固有细胞膜神经节苷类。结合对神经节苷脂调节整合素受体功能的观察，这些数据表明神经节苷脂可能在控制人类胶质瘤细胞的粘附性和侵袭性方面发挥核心作用。
Adhesion of eight cell lines, derived from human gliomas of different histological types, to fibronectin, collagen I, vitronectin, and laminin was investigated in vitro. The glioma cell lines were found to attach to these substrates to different extents. Interestingly, all cell lines strongly attached to laminin. In addition, glioma cell adhesion was found to be dose dependent. Moreover, adhesion of three cell lines to fibronectin and collagen I was partially inhibited and to vitronectin completely prevented by GRGDTP peptide, indicating the involvement of integrin receptors in glioma cell adhesion. We have demonstrated, recently, that gangliosides play an important role in promoting glioma cell invasion of the reconstituted basement membrane, Matrigel, in vitro. In order to study the mechanism of action of gangliosides in this process, the role of six gangliosides (GM1, GM3, GD3, GD1a, GD1b, and GT1b) in cell adhesion to the four proteins was investigated in three cell lines. Although all gangliosides, with the exception of GM3, were found to enhance cell adhesion to these proteins to different extents, GD3 proved to be the most effective adhesion-promoting ganglioside in all three cell lines. GM3 was found to inhibit cell adhesion to the four proteins in one cell line but enhanced cell adhesion in two other cell lines. The three cell lines were found to express both GD3 and gangliosides recognised by the A2B5 antibody. Furthermore, adhesion of the three cell lines to fibronectin, vitronectin, laminin, and collagen I was inhibited by incubation with A2B5, demonstrating the involvement of intrinsic cell membrane gangliosides in adhesion of glioma cells to these proteins. Taken together with the observation that gangliosides modulate integrin receptor function, these data suggest that gangliosides may play a central role in the control of the adhesive and invasive properties of human glioma cells.
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神经节苷脂和糖蛋白纤连蛋白受体参与细胞粘附纤连蛋白。
影响因子：
3.7
作者：
Stallcup,WB
通讯作者：
Stallcup,WB
影响因子：
15.9
作者：
GLADSON, CL;CHERESH, DA
通讯作者：
CHERESH, DA
人神经母细胞瘤细胞的神经节苷脂依赖性粘附事件受 RGDS 依赖性纤连蛋白受体和蛋白聚糖调节。
影响因子：
3.7
作者：
Mugnai,G;Lewandowska,K;Choi,HU;Rosenberg,LC;Culp,LA
通讯作者：
Culp,LA
单克隆抗体 A2B5 与神经节苷脂的结合。
DOI：
10.1016/s0006-291x(83)80218-6
发表时间：
1983
影响因子：
3.1
作者：
Kundu,SK;Pleatman,MA;Redwine,WA;Boyd,AE;Marcus,DM
通讯作者：
Marcus,DM
DOI：
--
发表时间：
1989
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
McKeever,PE;Fligiel,SE;Varani,J;Castle,RL;Hood,TW
通讯作者：
Hood,TW