The tubulin cofactor A is involved in hyphal growth, conidiation and cold sensitivity in Fusarium asiaticum.
The tubulin cofactor A is involved in hyphal growth, conidiation and cold sensitivity in Fusarium asiaticum.
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微管蛋白辅助因子 A 参与亚洲镰刀菌的菌丝生长、分生孢子形成和冷敏感性
DOI：
10.1186/s12866-015-0374-z
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发表时间：
2015-02-18
期刊：
影响因子：
4.2
通讯作者：
Ma Z
中科院分区：
文献类型：
--
作者：
Zhang X;Chen X;Jiang J;Yu M;Yin Y;Ma Z
微管蛋白辅因子A（tubulin cofactor A，TBCA）是微管蛋白辅因子的成员之一，在酿酒酵母（Saccharomycescerevisiae）中通过参与α/β微管蛋白异二聚体的折叠，对微管的功能起着重要的作用。然而，关于TBCA在丝状真菌中的作用知之甚少。在这项研究中，我们的特点是TBCA直系同源FaTBCA在亚洲镰刀菌。FaTBCA的缺失导致菌丝生长显着减少和分生孢子异常。FaTBCA缺失突变体（ΔFaTBCA-3）对低温的敏感性增加，甚至在4°C下失去生长能力。显微镜观察发现，ΔFaTBCA-3的菌丝在25 ℃至4 ℃下培养1或3天后表现出起泡表型，在4 ℃下培养3天后，约72%的膨大节含有多个核。然而，在4°C下孵育的野生型菌丝与在25°C下孵育的那些菌丝在表型上无法区分。这些结果表明，FaTBCA参与了冷胁迫（4°C）下F.亚洲人。出乎意料的是，ΔFaTBCA-3并未表现出对抗微管药物多菌灵的敏感性增加，尽管定量实时测定显示，用多菌灵处理后，FaTBCA的表达上调。此外，致病性测定结果表明，ΔFaTBCA-3对小麦穗部和非寄主番茄的致病力均有所降低。综上所述，本研究结果表明，FaTBCA在营养生长，分生孢子形成，温度敏感性和毒力的F。亚洲人。本文的在线版本（doi：10.1186/s12866-015-0374-z）包含补充材料，可供授权用户使用。
Tubulin cofactor A (TBCA), one of the members of tubulin cofactors, is of great importance in microtubule functions through participating in the folding of α/β-tubulin heterodimers in Saccharomyces cerevisiae. However, little is known about the roles of TBCA in filamentous fungi. In this study, we characterized a TBCA orthologue FaTBCA in Fusarium asiaticum. The deletion of FaTBCA caused dramatically reduced mycelial growth and abnormal conidiation. The FaTBCA deletion mutant (ΔFaTBCA-3) showed increased sensitivity to low temperatures and even lost the ability of growth at 4°C. Microscopic observation found that hyphae of ΔFaTBCA-3 exhibited blebbing phenotypes after shifting from 25 to 4°C for 1- or 3-day incubation and approximately 72% enlarged nodes contained several nuclei after 3-day incubation at 4°C. However, hyphae of the wild type incubated at 4°C were phenotypically indistinguishable from those incubated at 25°C. These results indicate that FaTBCA is involved in cell division under cold stress (4°C) in F. asiaticum. Unexpectedly, ΔFaTBCA-3 did not exhibit increased sensitivity to the anti-microtubule drug carbendazim although quantitative real-time assays showed that the expression of FaTBCA was up-regulated after treatment with carbendazim. In addition, pathogenicity assays showed that ΔFaTBCA-3 exhibited decreased virulence on wheat head and on non-host tomato. Taken together, results of this study indicate that FaTBCA plays crucial roles in vegetative growth, conidiation, temperature sensitivity and virulence in F. asiaticum. The online version of this article (doi:10.1186/s12866-015-0374-z) contains supplementary material, which is available to authorized users.
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