A high-coverage artificial chromosome library for the genome-wide screening of drug-resistance genes in malaria parasites.
A high-coverage artificial chromosome library for the genome-wide screening of drug-resistance genes in malaria parasites.
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DOI：
10.1101/gr.124164.111
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发表时间：
2012-05
期刊：
影响因子：
7
通讯作者：
Yuda M
中科院分区：
文献类型：
--
作者：
Iwanaga S;Kaneko I;Yuda M
抗药性寄生虫的全球传播是疟疾治疗的一个严重问题。虽然鉴定耐药基因对于对抗耐药寄生虫的努力至关重要，但尚未开发出有效的方法。在这里，我们报告了一个强大的方法，通过使用疟原虫人工染色体（PAC）鉴定寄生虫的抗性基因。将来自耐药啮齿类疟疾寄生虫伯氏疟原虫的大基因组DNA片段（10-50 kb）连接到PAC中并直接引入药物敏感的（即，野生型）寄生虫，产生包含寄生虫的整个基因组序列的PAC文库。随后，通过药物筛选选择获得抗性的转化寄生虫，并成功鉴定PAC中的抗性基因。此外，从抗乙胺嘧啶疟原虫的PAC文库中鉴定了耐药基因，进一步证明了我们方法的实用性。该方法将促进耐药基因的鉴定，为全球抗击耐药寄生虫做出贡献。
The global spread of drug-resistant parasites is a serious problem for the treatment of malaria. Although identifying drug-resistance genes is crucial for the efforts against resistant parasites, an effective approach has not yet been developed. Here, we report a robust method for identifying resistance genes from parasites by using a Plasmodium artificial chromosome (PAC). Large genomic DNA fragments (10–50 kb) from the drug-resistant rodent malaria parasite Plasmodium berghei were ligated into the PAC and directly introduced into the drug-sensitive (i.e., wild-type) parasite by electroporation, resulting in a PAC library that encompassed the whole genomic sequence of the parasite. Subsequently, the transformed parasites that acquired resistance were selected by screening with the drug, and the resistance gene in the PAC was successfully identified. Furthermore, the drug-resistance gene was identified from a PAC library that was made from the pyrimethamine-resistant parasite Plasmodium chabaudi, further demonstrating the utility of our method. This method will promote the identification of resistance genes and contribute to the global fight against drug-resistant parasites.
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DOI：
10.1126/science.1193225
发表时间：
2010-09-03
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Rottmann M;McNamara C;Yeung BK;Lee MC;Zou B;Russell B;Seitz P;Plouffe DM;Dharia NV;Tan J;Cohen SB;Spencer KR;González-Páez GE;Lakshminarayana SB;Goh A;Suwanarusk R;Jegla T;Schmitt EK;Beck HP;Brun R;Nosten F;Renia L;Dartois V;Keller TH;Fidock DA;Winzeler EA;Diagana TT
通讯作者：
Diagana TT
影响因子：
64.8
作者：
Carlton, Jane M.;Adams, John H.;Silva, Joana C.;Bidwell, Shelby L.;Lorenzi, Hernan;Caler, Elisabet;Crabtree, Jonathan;Angiuoli, Samuel V.;Merino, Emilio F.;Amedeo, Paolo;Cheng, Qin;Coulson, Richard M. R.;Crabb, Brendan S.;del Portillo, Hernando A.;Essien, Kobby;Feldblyum, Tamara V.;Fernandez-Becerra, Carmen;Gilson, Paul R.;Gueye, Amy H.;Guo, Xiang;Kang'a, Simon;Kooij, Taco W. A.;Korsinczky, Michael;Meyer, Esmeralda V. -S.;Nene, Vish;Paulsen, Ian;White, Owen;Ralph, Stuart A.;Ren, Qinghu;Sargeant, Tobias J.;Salzberg, Steven L.;Stoeckert, Christian J.;Sullivan, Steven A.;Yamamoto, Marcio M.;Hoffman, Stephen L.;Wortman, Jennifer R.;Gardner, Malcolm J.;Galinski, Mary R.;Barnwell, John W.;Fraser-Liggett, Claire M.
通讯作者：
Fraser-Liggett, Claire M.
影响因子：
14.9
作者：
Deitsch, KW;Driskill, CL;Wellems, TE
通讯作者：
Wellems, TE
DOI：
10.1073/pnas.88.8.3382
发表时间：
1991-04-01
影响因子：
11.1
作者：
WELLEMS, TE;WALKERJONAH, A;PANTON, LJ
通讯作者：
PANTON, LJ
DOI：
10.1073/pnas.94.4.1124
发表时间：
1997-02-18
影响因子：
11.1
作者：
Sirawaraporn, W;Sathitkul, T;Santi, DV
通讯作者：
Santi, DV