Procedure for Handling and Storage of Onchocerca volvulus Microfilariae Obtained from Skin Snips for Downstream Genetic Work.
Procedure for Handling and Storage of Onchocerca volvulus Microfilariae Obtained from Skin Snips for Downstream Genetic Work.
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DOI：
10.3390/tropicalmed8090445
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发表时间：
2023-09-12
影响因子：
2.9
通讯作者：
Kuesel AC
中科院分区：
文献类型：
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作者：
Hedtke SM;Kode A;Ukety TO;Mande JL;Abhafule GM;Raciu AA;Uvon CB;Jada SR;Hotterbeekx A;Siewe Fodjo JN;Mitreva M;Sebit W;Colebunders R;Grant WN;Kuesel AC
WHO and endemic countries target elimination of transmission of Onchocerca volvulus, the parasite causing onchocerciasis. Population genetic analysis of O. volvulus may provide data to improve the evidence base for decisions on when, where, and for how long to deploy which interventions and post-intervention surveillance to achieve elimination. Development of necessary methods and tools requires parasites suitable for genetic analysis. Based on our experience with microfilariae obtained from different collaborators, we developed a microfilariae transfer procedure for large-scale studies in the Democratic Republic of Congo (DRC) comparing safety and efficacy of ivermectin, the mainstay of current onchocerciasis elimination strategies, and moxidectin, a new drug. This procedure is designed to increase the percentage of microfilariae in skin snips suitable for genetic analysis, improve assignment to metadata, and minimize time and materials needed by the researchers collecting the microfilariae. Among 664 microfilariae from South Sudan, 35.7% and 39.5% failed the mitochondrial and nuclear qPCR assay. Among the 576 microfilariae from DRC, 16.0% and 16.7% failed these assays, respectively. This difference may not only be related to the microfilariae transfer procedure but also to other factors, notably the ethanol concentration in the tubes in which microfilariae were stored (64% vs. ≥75%).
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影响因子：
3.8
作者：
Michalski ML;Griffiths KG;Williams SA;Kaplan RM;Moorhead AR
通讯作者：
Moorhead AR
影响因子：
3.8
作者：
Fodjo, Joseph Nelson Siewe;Mandro, Michel;Colebunders, Robert
通讯作者：
Colebunders, Robert
影响因子：
56.3
作者：
Chesnais, Cedric B.;Nana-Djeunga, Hugues C.;Boussinesq, Michel
通讯作者：
Boussinesq, Michel
DOI：
10.3390/pathogens10020099
发表时间：
2021-01-21
期刊：
Pathogens (Basel, Switzerland)
影响因子：
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作者：
Shey RA;Ghogomu SM;Shintouo CM;Nkemngo FN;Nebangwa DN;Esoh K;Yaah NE;Manka'aFri M;Nguve JE;Ngwese RA;Njume FN;Bertha FA;Ayong L;Njemini R;Vanhamme L;Souopgui J
通讯作者：
Souopgui J
DOI：
10.3390/pathogens12070971
发表时间：
2023-07-24
期刊：
Pathogens (Basel, Switzerland)
影响因子：
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作者：
通讯作者：
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