Melon yellow-green plant (Cmygp) encodes a Golden2-like transcription factor regulating chlorophyll synthesis and chloroplast development
Melon yellow-green plant (Cmygp) encodes a Golden2-like transcription factor regulating chlorophyll synthesis and chloroplast development
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瓜黄绿色植物（Cmygp）编码类Golden2转录因子，调节叶绿素合成和叶绿体发育
DOI：
10.1007/s00122-023-04343-9
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发表时间：
2023-03
影响因子：
5.4
通讯作者：
Yang Luming
中科院分区：
文献类型：
--
作者：
Yang Sen;Wang Xiaojuan;Yan Wenkai;Zhang Yu;Song Pengyao;Guo Yaomiao;Xie Kuixi;Hu Jianbin;Hou Juan;Wu Yufeng;Zhu Huayu;Sun Shouru;Yang Luming
编码Golden 2类转录因子的CmYGP基因的SNP突变是甜瓜黄绿色植株性状的原因。叶绿素是植物和人类健康所必需的有益物质。明确叶绿素的调控网络是提高水果营养品质的必要条件。在不同的甜瓜品种中已经鉴定出至少6个黄化基因，但没有一个被克隆，并且甜瓜中叶绿素合成和叶绿体发育的分子机制仍然不清楚。在此，NSL 73046，一个黄绿色植物（Cmygp）突变体，使第一个黄化基因在甜瓜的图位克隆。CmYGP编码一个Golden 2样转录因子。时空表达分析证实了CmYGP在所有绿色组织中的高表达，特别是在幼叶和果皮中。病毒诱导的基因沉默和分子标记辅助选择培育的近等基因系进一步证实了CmYGP的下调可以减少叶绿体数量和叶绿素含量，从而导致甜瓜叶片和果实黄绿色，而CmYGP在番茄中的过表达也导致叶片和果实深绿色。RNA-seq分析表明，CmYGP显著影响了叶绿体发育相关关键基因的表达。综上所述，这些结果表明，CmYGP调节叶绿素合成和叶绿体发育，从而影响甜瓜果实发育。本研究也为提高甜瓜果实品质提供了新的策略。
A SNP mutation in CmYGP gene encoding Golden2-like transcription factor is responsible for melon yellow-green plant trait. Chlorophylls are essential and beneficial substances for both plant and human health. Identifying the regulatory network of chlorophyll is necessary to improve the nutritional quality of fruits. At least six etiolation genes have been identified in different melon varieties, but none of them have been cloned, and the molecular mechanisms underlying chlorophyll synthesis and chloroplast development in melon remain unclear. Here, the NSL73046, a yellow-green plant (Cmygp) mutant, enabled the map-based cloning of the first etiolation gene in melon. CmYGP encodes a Golden2-like transcription factor. Spatiotemporal expression analyses confirmed the high CmYGP expression in all green tissues, particularly in young leaves and fruit peels. Virus-induced gene silencing and the development of near-isogenic line by marker-assisted selection further confirmed that downregulation of CmYGP can reduce chloroplast number and chlorophyll content, thereby resulting in yellow-green leaves and fruits in melon, and overexpression of CmYGP in tomatoes also led to dark-green leaves and fruits. RNA-seq analysis revealed that CmYGP greatly affected the expression of key genes associated with chloroplast development. Taken together, these findings demonstrated that CmYGP regulate chlorophyll synthesis and chloroplast development thus affect fruit development in melon. This study also offers a new strategy to enhance fruit quality in melon.
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