Mapping and functional verification of leaf yellowing genes in watermelon during whole growth period.
Mapping and functional verification of leaf yellowing genes in watermelon during whole growth period.
复制标题
在整个生长期间，西瓜中叶片黄基因的映射和功能验证。
DOI：
10.3389/fpls.2022.1049114
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发表时间：
2022
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
提高光能利用效率是西瓜增产、改善品质的有效途径。叶子是光合作用的主要场所，叶子的颜色与光合作用的变化直接相关。此外，叶片黄化可作为标记性状，在西瓜杂交育种、提高种子育种中发挥重要作用。它不仅可用于消除苗期杂种，还可用于测定种子纯度。本研究首先利用全生育期叶黄化西瓜突变体w-yl和自交系ZK进行转录组分析，鉴定出对照组w-yl-vs-ZK中的2,471个差异表达基因（DEG）。在基因本体（GO）富集途径的前20个术语中，有17个术语与光合作用相关。 KEGG通路富集分析表明，最丰富的通路是光合作用——天线蛋白。 F2群体是通过与自交系ZK常规杂交构建的。遗传分析表明，该突变体的叶片黄化是由单个隐性基因控制的。通过indel特异性PCR标记，西瓜叶片黄化基因位于2号染色体Ind14,179,011和InD16,396,362之间，区域大小为2.217 Mb。区间内发现5个基因可能存在w-yl基因片段缺失，其中Cla97C02G036010、Cla97C02G036030、Cla97C02G036040、Cla97C02G036050为全片段缺失，Cla97C02G0360为C端部分碱基缺失。基因功能验证结果表明，Cla97C02G036040、Cla97C02G036050和Cla97C02G036060可能是导致w-yl叶片黄化的关键因素。
Increasing light energy utilization efficiency is an effective way to increase yield and improve quality of watermelon. Leaf is the main place for photosynthesis, and the color of leaf is directly related to the change of photosynthesis. In addition, leaf yellowing can be used as a marker trait to play an important role in watermelon hybrid breeding and improve seed breeding. It can not only be used to eliminate hybrids at seedling stage, but also be used to determine seed purity. In this study, transcriptome analysis was first carried out using the whole growth period leaf yellowing watermelon mutant w-yl and inbred line ZK, and identified 2,471 differentially expressed genes (DEGs) in the comparison group w-yl-vs-ZK. Among the top 20 terms of the gene ontology (GO) enrichment pathway, 17 terms were related to photosynthesis. KEGG pathway enrichment analysis showed that the most abundant pathway was photosynthesis—antenna proteins. The F2 population was constructed by conventional hybridization with the inbred line ZK. Genetic analysis showed that leaf yellowing of the mutant was controlled by a single recessive gene. The leaf yellowing gene of watermelon located between Ind14,179,011 and InD16,396,362 on chromosome 2 by using indel-specific PCR markers, with a region of 2.217 Mb. In the interval, it was found that five genes may have gene fragment deletion in w-yl, among which Cla97C02G036010, Cla97C02G036030, Cla97C02G036040, Cla97C02G036050 were the whole fragment loss, and Cla97C02G0360 was the C-terminal partial base loss. Gene function verification results showed that Cla97C02G036040, Cla97C02G036050 and Cla97C02G036060 may be the key factors leading to yellowing of w-yl leaves.
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影响因子：
6.4
作者：
DEI, M
通讯作者：
DEI, M
影响因子：
3.7
作者：
Huang, Jin-Ling;Sun, Gui-Ling;Zhang, Da-Ming
通讯作者：
Zhang, Da-Ming
影响因子：
5.1
作者：
Gothandam, KM;Kim, ES;Chung, YY
通讯作者：
Chung, YY
影响因子：
2.7
作者：
Hibara, Ken-ichiro;Obara, Mari;Nagato, Yasuo
通讯作者：
Nagato, Yasuo
影响因子：
--
作者：
Chen, Ting-ting;Fu, Wei-meng;Tao, Long-xing
通讯作者：
Tao, Long-xing