Characterization of two infection-induced transcription factors of Magnaporthe oryzae reveals their roles in regulating early infection and effector expression.
Characterization of two infection-induced transcription factors of Magnaporthe oryzae reveals their roles in regulating early infection and effector expression.
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稻瘟病菌两种感染诱导转录因子的表征揭示了它们在调节早期感染和效应子表达中的作用
DOI：
10.1111/mpp.13224
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发表时间：
2022-08
影响因子：
4.9
通讯作者：
Li, Ya
中科院分区：
文献类型：
--
作者：
Cao, Yiyang;Chen, Jia;Xie, Xuze;Liu, Shenghua;Jiang, Yue;Pei, Mengtian;Wu, Qianfei;Qi, Pengfei;Du, Lili;Peng, Baoyi;Lan, Jianwu;Wu, Fan;Feng, Ke;Zhang, Yifei;Fang, Yu;Liu, Muxing;Jaber, Mohammed Y.;Wang, Zonghua;Olsson, Stefan;Lu, Guodong;Li, Ya
稻瘟病菌（Magnaporthe oryzae）感染的初始阶段，即接种后36小时之前，是利用病原体效应物克服寄主防御并最终引起疾病的关键时间。然而，这一过程在转录水平上是如何调控的，在很大程度上仍然是未知的。本研究对两个m.o ryzae早期感染诱导转录因子基因（MOEITF1和MOEITF2）进行了功能表征，并分析了它们在这一过程中的作用。靶基因缺失和突变体表型分析表明，突变体Δmoeitf1和Δmoeitf2仅在侵染生长方面存在缺陷，而在营养生长、无性/有性产孢、分生孢子萌发和附着胞形成方面没有缺陷。对30个可能的效应基因的基因表达分析显示，大多数效应基因在突变体中下调，这意味着可能受到转录因子的调控。在突变体中人工过表达两种严重下调的效应物T1REP和T2REP，分别部分恢复了Δmoeitf1和Δmoeitf2的致病性，表明它们受到直接调控。酵母单杂交实验和电泳迁移率转移实验表明，Moeitf1特异性结合T1REP启动子，Moeitf2特异性结合T2REP启动子。T1REP和T2REP在感染过程中均有分泌，T2REP突变体致病性严重降低。我们的研究结果表明，真菌特异性的Moeitf1和Moeitf2转录因子在水稻m.o ryzae穿透水稻表皮细胞后早期建立的一个重要步骤中发挥了关键作用，强调了这些可能是疾病控制的靶点。
The initial stage of rice blast fungus, Magnaporthe oryzae, infection, before 36 h postinoculation, is a critical timespan for deploying pathogen effectors to overcome the host's defences and ultimately cause the disease. However, how this process is regulated at the transcription level remains largely unknown. This study functionally characterized two M. oryzae Early Infection‐induced Transcription Factor genes (MOEITF1 and MOEITF2) and analysed their roles in this process. Target gene deletion and mutant phenotype analysis showed that the mutants Δmoeitf1 and Δmoeitf2 were only defective for infection growth but not for vegetative growth, asexual/sexual sporulation, conidial germination, and appressoria formation. Gene expression analysis of 30 putative effectors revealed that most effector genes were down‐regulated in mutants, implying a potential regulation by the transcription factors. Artificial overexpression of two severely down‐regulated effectors, T1REP and T2REP, in the mutants partially restored the pathogenicity of Δmoeitf1 and Δmoeitf2, respectively, indicating that these are directly regulated. Yeast one‐hybrid assay and electrophoretic mobility shift assay indicated that Moeitf1 specifically bound the T1REP promoter and Moeitf2 specifically bound the T2REP promoter. Both T1REP and T2REP were predicted to be secreted during infection, and the mutants of T2REP were severely reduced in pathogenicity. Our results indicate crucial roles for the fungal‐specific Moeitf1 and Moeitf2 transcription factors in regulating an essential step in M. oryzae early establishment after penetrating rice epidermal cells, highlighting these as possible targets for disease control.
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DOI：
10.1126/science.aau5480
发表时间：
2020-05-01
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Lazzaro BP;Zasloff M;Rolff J
通讯作者：
Rolff J
生长素样蛋白MoSwa2作为稻瘟病菌Magnaporthe oryzae的网格蛋白脱衣因子促进效应子分泌和毒力
DOI：
10.1111/nph.17181
发表时间：
2021-04
期刊：
The New phytologist
影响因子：
--
作者：
Liu M;Hu J;Zhang A;Dai Y;Chen W;He Y;Zhang H;Zheng X;Zhang Z
通讯作者：
Zhang Z
稻瘟菌真菌发育和植物感染所需的电子传递黄素蛋白及其脱氢酶的功能表征
影响因子：
4.6
作者：
Li Y;Zhu J;Hu J;Meng X;Zhang Q;Zhu K;Chen X;Chen X;Li G;Wang Z;Lu G
通讯作者：
Lu G
影响因子：
5.6
作者：
Jwa NS;Hwang BK
通讯作者：
Hwang BK
影响因子：
4.5
作者：
Kim S;Park SY;Kim KS;Rho HS;Chi MH;Choi J;Park J;Kong S;Park J;Goh J;Lee YH
通讯作者：
Lee YH