Vegetative compatibility groups partition variation in the virulence of Verticillium dahliae on strawberry.
Vegetative compatibility groups partition variation in the virulence of Verticillium dahliae on strawberry.
复制标题
DOI：
10.1371/journal.pone.0191824
复制
发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Harrison RJ
中科院分区：
文献类型：
--
作者：
Fan R;Cockerton HM;Armitage AD;Bates H;Cascant-Lopez E;Antanaviciute L;Xu X;Hu X;Harrison RJ
草莓黄萎病菌(Fragaria X Ananassa)侵染草莓是引起世界各地土生草莓枯萎的主要原因。为了了解病原体的哪些成分正在影响疾病的表达，我们在大丽轮枝菌分离物的样本中筛选了已知效应器VdAve1的存在。来自草莓的分离物被发现含有VdAve1，并根据其营养亲和性群(VCG)分为两个主要分支；英国草莓分离物没有含有VdAve1。Vc分支与其毒力水平密切相关。在两个分支中都发现了含有VdAve1的草莓病原菌，这与最近发表的一些发现形成了鲜明的对比。在草莓上，含有VdAve1的分离物在感染早期具有较高的致病力，随着侵染程度的加深，其致病力减弱。将含有VdAve1的非VdAve1致病分离物转化到感病草莓品种上，既不能提高也不能降低致病力。因此，在大丽叶霉菌谱系中，存在对VdAve1具有上位性的毒力因子，并可能有多条独立的途径获得草莓的高毒力。两个VCGS分离株的基因组测序结果表明，两个VCGS分离株在全基因组水平上是分化的，并且在推测的效应物含量上存在多重变化，这表明不同的VCGS可能进化出了不同的侵染草莓的策略，导致了致病力测试中不同的毒力水平。因此，重要的是同时考虑克隆谱系和效应器互补，因为每个谱系的适应潜力将不同，即使它们包含相同的种族决定效应器。
Verticillium dahliae infection of strawberry (Fragaria x ananassa) is a major cause of disease-induced wilting in soil-grown strawberries across the world. To understand what components of the pathogen are affecting disease expression, the presence of the known effector VdAve1 was screened in a sample of Verticillium dahliae isolates. Isolates from strawberry were found to contain VdAve1 and were divided into two major clades, based upon their vegetative compatibility groups (VCG); no UK strawberry isolates contained VdAve1. VC clade was strongly related to their virulence levels. VdAve1-containing isolates pathogenic on strawberry were found in both clades, in contrast to some recently published findings. On strawberry, VdAve1-containing isolates had significantly higher virulence during early infection, which diminished in significance as the infection progressed. Transformation of a virulent non-VdAve1 containing isolate, with VdAve1 was found neither to increase nor decrease virulence when inoculated on a susceptible strawberry cultivar. There are therefore virulence factors that are epistatic to VdAve1 and potentially multiple independent routes to high virulence on strawberry in V. dahliae lineages. Genome sequencing a subset of isolates across the two VCGs revealed that isolates were differentiated at the whole genome level and contained multiple changes in putative effector content, indicating that different clonal VCGs may have evolved different strategies for infecting strawberry, leading to different virulence levels in pathogenicity tests. It is therefore important to consider both clonal lineage and effector complement as the adaptive potential of each lineage will differ, even if they contain the same race determining effector.
登录
查看更多内容
影响因子：
2.7
作者：
Collado-Romero, M.;Berbegal, M.;Mercado-Blanco, J.
通讯作者：
Mercado-Blanco, J.
影响因子：
10.7
作者：
Bertels F;Silander OK;Pachkov M;Rainey PB;van Nimwegen E
通讯作者：
van Nimwegen E
DOI：
10.1126/science.1171647
发表时间：
2009-05-08
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Boller T;He SY
通讯作者：
He SY
影响因子：
1.7
作者：
Bankevich, Anton;Nurk, Sergey;Pevzner, Pavel A.
通讯作者：
Pevzner, Pavel A.
影响因子：
4.5
作者：
Bhat, RG;Smith, RF;Subbarao, KV
通讯作者：
Subbarao, KV