Micro-crystallography comes of age.

Micro-crystallography comes of age.
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DOI:
10.1016/j.sbi.2012.09.001
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发表时间:
2012-10
影响因子:
6.8
通讯作者:
Yamamoto M
Yamamoto M
中科院分区:
生物学2区
文献类型:
--
作者:
Smith JL;Fischetti RF;Yamamoto M

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大分子结晶学的最新革命是由专用的、用户友好的微晶学光束线的开发引发的。直径为20微米或更小的耀眼X射线束,现在大多数同步加速器源都可以使用,可以从以前无法获得的样品中确定结构。与传统的结晶学相比,具有一个或多个小尺寸的晶体在用适当匹配的光束尺寸记录时具有极大地改善信噪比的衍射图案。结构可以从非均匀样品中孤立的、良好的衍射区中解算出来。本文综述了产生微束的技术要求和方法,以及它们如何继续改变结晶学的实践。
The latest revolution in macromolecular crystallography was incited by the development of dedicated, user friendly, micro-crystallography beamlines. Brilliant X-ray beams of diameter 20 microns or less, now available at most synchrotron sources, enable structure determination from samples that previously were inaccessible. Relative to traditional crystallography, crystals with one or more small dimensions have diffraction patterns with vastly improved signal-to-noise when recorded with an appropriately matched beam size. Structures can be solved from isolated, well diffracting regions within inhomogeneous samples. This review summarizes the technological requirements and approaches to producing micro-beams and how they continue to change the practice of crystallography.
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