DNA inserted two bases down from the initiation site of a SP6 polymerase transcription vector is transcribed efficiently in vitro.
DNA inserted two bases down from the initiation site of a SP6 polymerase transcription vector is transcribed efficiently in vitro.
复制标题
从 SP6 聚合酶转录载体起始位点向下插入两个碱基的 DNA 可在体外有效转录。
DOI:
10.1093/nar/15.3.1337
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发表时间:
1987
影响因子:
14.9
通讯作者:
Kaesberg,P
中科院分区:
文献类型:
--
作者:
Bujarski,JJ;Kaesberg,P
Figure 1. A. Polylinker sequences inoriginal pGEM3 (and pJMSP-0) and in the new pJMSP-8 plasmids. Vector sequences are in bold letters; the first transcribed base is underlined. B. Electrophoretic analysis (1.5% agarose) of SP6 RNA polymerasetranscripts (their 5'end sequences are shown in brackets): lane 1. Plasmid pJMSP-8, linearized with BglII (GGCCTGC); lane 2. Plasmid pJMSP-0, linearized with BglII (GAATACA); lanes 3-6. Plasmid pJMSP-8, having, respectively, the following brome mosaic virus cDNA1 (4) fragments insertedbetween the StuI-EcoRI sites and linearized with EcoRI: SnaBI/EcoRI (GGGTAGG), EcoRV/EcoRI (GGATCAC), BalI/EcoRI (GGCCAAC) and BclI/EcoRI (GGGATCA).
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影响因子:
11.2
作者:
I. Fidler;I. Hart
通讯作者:
I. Hart
DOI:
--
发表时间:
1983
期刊:
Invasion & metastasis
影响因子:
--
作者:
F. Miller;Miller Be;G. Heppner
通讯作者:
F. Miller;Miller Be;G. Heppner
影响因子:
11.2
作者:
Stackpole,CW
通讯作者:
Stackpole,CW
DOI:
10.1093/jnci/67.4.947
发表时间:
1981
期刊:
Journal of the National Cancer Institute
影响因子:
--
作者:
I. J. Fidler;Eilene Gruys;Maria A. Cifone;Zoa L. Barnes;C. Bucana
通讯作者:
C. Bucana
影响因子:
56.9
作者:
I. Fidler;Richard J White
通讯作者:
Richard J White