Multiple Cytochrome P450 genes: their constitutive overexpression and permethrin induction in insecticide resistant mosquitoes, Culex quinquefasciatus.
Multiple Cytochrome P450 genes: their constitutive overexpression and permethrin induction in insecticide resistant mosquitoes, Culex quinquefasciatus.
复制标题
DOI：
10.1371/journal.pone.0023403
复制
发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Zhang L
中科院分区：
文献类型：
--
作者：
Liu N;Li T;Reid WR;Yang T;Zhang L
从致倦库蚊中分离到4个细胞色素P450 cDNA，分别为CYP6AA7、CYP9J40、CYP9J34和CYP9M10。比较了具有不同抗性表型的三个不同抗性种群的P450基因表达和氯菊酯的诱导作用，从敏感(S-Lab)、中间(HAmCqG0，田间亲本种群)到高抗性(HAmCqG8，二氯苯菊酯精选后代HAmCqG0)。在蚊虫幼虫阶段，P450基因的表达与蚊虫对氯氰菊酯的抗性水平和选择氯氰菊酯后的水平有很强的相关性，其中HAmCqG8的表达水平最高，表明CYP6AA7、CYP9J40、CYP9J34和CYP9M10在蚊虫幼虫对氯氰菊酯的抗性中起着重要的作用。只有CYP6AA7在HAmCqG8成蚊中有显著的过表达。其他P450基因在受试蚊虫种群中的表达水平相似，表明不同的P450基因可能参与了不同发育阶段对杀虫剂压力的反应。氯氰菊酯还能进一步诱导抗性蚊体的细胞色素P6AA7、细胞色素P9J34和细胞色素P9M10的表达。综上所述，这些结果表明，在抗药性蚊子中，多个P450基因通过结构性过表达和诱导机制上调，从而提高了P450基因的整体表达水平。
Four cytochrome P450 cDNAs, CYP6AA7, CYP9J40, CYP9J34, and CYP9M10, were isolated from mosquitoes, Culex quinquefasciatus. The P450 gene expression and induction by permethrin were compared for three different mosquito populations bearing different resistance phenotypes, ranging from susceptible (S-Lab), through intermediate (HAmCqG0, the field parental population) to highly resistant (HAmCqG8, the 8th generation of permethrin selected offspring of HAmCqG0). A strong correlation was found for P450 gene expression with the levels of resistance and following permethrin selection at the larval stage of mosquitoes, with the highest expression levels identified in HAmCqG8, suggesting the importance of CYP6AA7, CYP9J40, CYP9J34, and CYP9M10 in the permethrin resistance of larva mosquitoes. Only CYP6AA7 showed a significant overexpression in HAmCqG8 adult mosquitoes. Other P450 genes had similar expression levels among the mosquito populations tested, suggesting different P450 genes may be involved in the response to insecticide pressure in different developmental stages. The expression of CYP6AA7, CYP9J34, and CYP9M10 was further induced by permethrin in resistant mosquitoes. Taken together, these results indicate that multiple P450 genes are up-regulated in insecticide resistant mosquitoes through both constitutive overexpression and induction mechanisms, thus increasing the overall expression levels of P450 genes.
登录
查看更多内容
影响因子：
2.7
作者：
Aerts, JL;Gonzales, MI;Topalian, SL
通讯作者：
Topalian, SL
影响因子：
3.8
作者：
Kasai, S;Weerashinghe, IS;Yamakawa, M
通讯作者：
Yamakawa, M
影响因子：
3.8
作者：
Itokawa, Kentaro;Komagata, Osamu;Tomita, Takashi
通讯作者：
Tomita, Takashi
影响因子：
3.8
作者：
CARINO, FA;KOENER, JF;FEYEREISEN, R
通讯作者：
FEYEREISEN, R
影响因子：
3.8
作者：
Komagata, Osamu;Kasai, Shinji;Tomita, Takashi
通讯作者：
Tomita, Takashi