A tough act to follow: collagen hydrogel modifications to improve mechanical and growth factor loading capabilities.
A tough act to follow: collagen hydrogel modifications to improve mechanical and growth factor loading capabilities.
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DOI：
10.1016/j.mtbio.2021.100098
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发表时间：
2021-03
期刊：
影响因子：
--
通讯作者：
Salmeron-Sanchez M
中科院分区：
文献类型：
--
作者：
Sarrigiannidis SO;Rey JM;Dobre O;González-García C;Dalby MJ;Salmeron-Sanchez M
胶原水凝胶因其低成本、低免疫原性、多功能性、生物相容性以及与天然细胞外基质的相似性而成为研究最充分的药物输送和原位组织工程平台之一。尽管胶原蛋白在很大程度上决定了天然结缔组织的拉伸性能，但在没有共价交联的情况下，胶原蛋白水凝胶的力学性能相对较低。当试图再生更坚硬和更坚固的天然组织(如骨骼)时，这尤其有问题。此外，与基于细胞外基质蛋白(如纤维连接蛋白)的水凝胶不同，胶原水凝胶没有任何生长因子(GF)特异性结合部位，通常不能隔离生理(少量)蛋白质。生长因子结合和原位呈现是决定细胞命运而不会引起恶性肿瘤组织生长等不良影响的特性，可以显著地帮助组织再生过程。为了缓解这些问题，研究人员开发了几种策略，通过物理或化学修饰来提高胶原水凝胶的机械性能。这可以扩大胶原水凝胶的适用性，使其适用于承受持续负荷的组织。通过直接加载、化学交联、静电相互作用和其他载体系统的开发，还从数学和实验上探索了玻璃纤维的输送。这篇全面的文章探索了在的胶原水凝胶系统中优化这些参数(机械性能和GF释放)的方法，并检测了它们的体外或体内生物学效应。因此，这篇文章可以是一个有用的工具，通过指导研究人员根据他们的胶原蛋白水凝胶设计要求找到合适的方向，从而简化该领域未来的研究。
Collagen hydrogels are among the most well-studied platforms for drug delivery and in situ tissue engineering, thanks to their low cost, low immunogenicity, versatility, biocompatibility, and similarity to the natural extracellular matrix (ECM). Despite collagen being largely responsible for the tensile properties of native connective tissues, collagen hydrogels have relatively low mechanical properties in the absence of covalent cross-linking. This is particularly problematic when attempting to regenerate stiffer and stronger native tissues such as bone. Furthermore, in contrast to hydrogels based on ECM proteins such as fibronectin, collagen hydrogels do not have any growth factor (GF)-specific binding sites and often cannot sequester physiological (small) amounts of the protein. GF binding and in situ presentation are properties that can aid significantly in the tissue regeneration process by dictating cell fate without causing adverse effects such as malignant tumorigenic tissue growth. To alleviate these issues, researchers have developed several strategies to increase the mechanical properties of collagen hydrogels using physical or chemical modifications. This can expand the applicability of collagen hydrogels to tissues subject to a continuous load. GF delivery has also been explored, mathematically and experimentally, through the development of direct loading, chemical cross-linking, electrostatic interaction, and other carrier systems. This comprehensive article explores the ways in which these parameters, mechanical properties and GF delivery, have been optimized in collagen hydrogel systems and examines their in vitro or in vivo biological effect. This article can, therefore, be a useful tool to streamline future studies in the field, by pointing researchers into the appropriate direction according to their collagen hydrogel design requirements.
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