On-chip DNA preconcentration in different media conductivities by electrodeless dielectrophoresis.
On-chip DNA preconcentration in different media conductivities by electrodeless dielectrophoresis.
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通过无电极介电电泳在不同的介质电导率中进行芯片上 DNA 预富集。
DOI：
10.1063/1.4932177
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发表时间：
2015
期刊：
影响因子：
3.2
通讯作者：
W. Wen
中科院分区：
文献类型：
--
作者：
Shunbo Li;Ziran Ye;Yu Sanna Hui;Yibo Gao;Yusheng Jiang;W. Wen
无电极介电泳因其简单、稳健且易于实施而成为实现纳米粒子和生物分子预富集的最佳选择。我们设计了一个简单的芯片，其间有微通道和纳米缝隙，然后研究了 DNA 在高导电介质和低导电介质中的捕获，分别对应于正介电泳 (DEP) 和负介电泳 (DEP)。研究不同电导率介质中的捕获非常重要，因为人们总是需要处理具有不同电导率的样品溶液。通过荧光强度的变化来分析捕获过程。结果表明，与现有方法相比，在较低的电场强度（10V/cm）下，DNA 可以被捕获在高导电介质和低导电介质的纳米缝隙中。这对于抑制DEP相关实验中的焦耳热效应来说是一个显着的改进。我们的工作可以让研究人员通过芯片实验室系统中的简单且低成本的设备捕获 DNA。
Electrodeless dielectrophoresis is the best choice to achieve preconcentration of nanoparticles and biomolecules due to its simple, robust, and easy implementation. We designed a simple chip with microchannels and nano-slits in between and then studied the trapping of DNA in high conductive medium and low conductive medium, corresponding to positive and negative dielectrophoresis (DEP), respectively. It is very important to investigate the trapping in media with different conductivities since one always has to deal with the sample solutions with different conductivities. The trapping process was analyzed by the fluorescent intensity changes. The results showed that DNA could be trapped at the nano-slit in both high and low conductive media in a lower electric field strength (10 V/cm) compared to the existing methods. This is a significant improvement to suppress the Joule heating effect in DEP related experiments. Our work may give insight to researchers for DNA trapping by a simple and low cost device in the Lab-on-a-Chip system.
影响因子：
3.4
作者：
Mai DJ;Brockman C;Schroeder CM
通讯作者：
Schroeder CM
影响因子：
3.9
作者：
Vlassiouk, I;Krasnoslobodtsev, A;Germann, M
通讯作者：
Germann, M