Method measuring oxygen tension and transport within subcutaneous devices.
Method measuring oxygen tension and transport within subcutaneous devices.
复制标题
测量皮下装置内氧张力和输送的方法。
DOI：
10.1117/1.jbo.19.8.087006
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发表时间：
2014
影响因子：
3.5
通讯作者：
Botvinick,Elliot
中科院分区：
文献类型：
--
作者：
Weidling,John;Sameni,Sara;Lakey,JonathanRT;Botvinick,Elliot
细胞疗法有望在疾病治疗中取代整个器官的植入。对于大多数细胞类型，体内存活力取决于氧气输送，以避免缺氧的毒性作用。一个有前途的方法是原位血管化的植入装置，可以调解缺氧和提高植入细胞和组织的寿命和效用。尽管周围组织中氧合的数学模型和大量测量已用于估计装置内的氧合，但此类估计不足以确定所供应的氧是否足以用于植入细胞和组织的整个厚度。我们已经开发了一种技术，其中氧敏感微粒（OSM）被纳入皮下植入装置的体积。这些设备内的氧分压可以通过放置在皮肤表面上的光学探针直接在体内测量。作为验证，OSM已被并入藻酸盐珠粒中，藻酸盐珠粒通常用作免疫隔离装置以包封胰岛细胞。将藻酸盐珠植入Sprague-Dawley大鼠的皮下空间。通过珠的氧气运输的特征在于从动态OSM信号响应于吸入氧气的变化。几天来氧气动力学的变化证明了我们技术的实用性。
Cellular therapies hold promise to replace the implantation of whole organs in the treatment of disease. For most cell types,in vivoviability depends on oxygen delivery to avoid the toxic effects of hypoxia. A promising approach is thein situvascularization of implantable devices which can mediate hypoxia and improve both the lifetime and utility of implanted cells and tissues. Although mathematical models and bulk measurements of oxygenation in surrounding tissue have been used to estimate oxygenation within devices, such estimates are insufficient in determining if supplied oxygen is sufficient for the entire thickness of the implanted cells and tissues. We have developed a technique in which oxygen-sensitive microparticles (OSMs) are incorporated into the volume of subcutaneously implantable devices. Oxygen partial pressure within these devices can be measured directlyin vivoby an optical probe placed on the skin surface. As validation, OSMs have been incorporated into alginate beads, commonly used as immunoisolation devices to encapsulate pancreatic islet cells. Alginate beads were implanted into the subcutaneous space of Sprague–Dawley rats. Oxygen transport through beads was characterized from dynamic OSM signals in response to changes in inhaled oxygen. Changes in oxygen dynamics over days demonstrate the utility of our technology.
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影响因子：
3.3
作者：
Lamb, Morgan;Laugenour, Kelly;Lakey, Jonathan R. T.
通讯作者：
Lakey, Jonathan R. T.
人和猪胰岛的分离以及猪胰岛移植。
DOI：
10.1016/0022-4804(74)90017-1
发表时间：
1974
期刊：
The Journal of surgical research
影响因子：
--
作者：
D. Sutherland;Michael W. Steffes;G.Eric Bauer;Darlene McManus;Bran D. Noe;J. Najarian
通讯作者：
J. Najarian
在纤维蛋白中培养的幼猪内分泌胰岛显示出对过氧化氢的抵抗力得到改善
影响因子：
2.2
作者：
C. Kuehn;J. Lakey;Morgan W. Lamb;P. Vermette
通讯作者：
P. Vermette
可在原位校准的植入式腹膜氧眼压计。
DOI：
--
发表时间：
1990
期刊：
ASAIO transactions
影响因子：
--
作者：
Spokane,RB;ClarkJr,LC;Bhargava,HK;Burden,MK;Davis,SL
通讯作者：
Davis,SL
用于胰岛封装和皮下移植的生物相容性藻酸盐的体内选择。
影响因子：
--
作者：
S. Vériter;Julien Mergen;R. Goebbels;Najima Aouassar;Charles Grégoire;B. Jordan;P. Lévêque;B. Gallez;P. Gianello;D. Dufrane
通讯作者：
D. Dufrane