Selenium deficiency impaired structural integrity of the head kidney, spleen and skin in young grass carp (Ctenopharyngodon idella)
Selenium deficiency impaired structural integrity of the head kidney, spleen and skin in young grass carp (Ctenopharyngodon idella)
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缺硒损害草鱼幼鱼（Ctenopharyngodon idella）头肾、脾脏和皮肤的结构完整性
DOI：
10.1016/j.fsi.2018.08.038
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发表时间：
2018-11
影响因子：
4.7
通讯作者：
Liu Y
中科院分区：
文献类型：
--
作者：
Zheng L;Jiang WD;Feng L;Wu P;Tang L;Kuang SY;Zeng YY;Zhou XQ;Liu Y
This study focused on the effects of dietary selenium deficiency on structural integrity of the head kidney, spleen and skin in young grass carp (Ctenopharyngodon idella). A total of 540 healthy grass carp (mean weight 226.48 ± 0.68 g) were randomly divided into six groups and fed six separate diets with graded dietary levels of selenium (0.025–1.049 mg/kg diet) for 80 days. Results showed that selenium deficiency (1) caused oxidative damage in part by reducing the activities of antioxidant enzymes (such as SOD, CAT, GPx, GST and GR) and glutathione (GSH) content, down-regulating the transcript abundances of antioxidant enzymes (exceptGSTp1) partly related to Kelch-like-ECH-associated protein 1a (Keap1a)/NF-E2-related factor 2 (Nrf2) signalling; (2) aggravated apoptosis in part by up-regulating the mRNA levels ofcaspase-2,-3,-7,-8and -9, which were partially related to p38MAPK/FasL/caspase-8 signalling and JNK/(BAX, Bcl-2, Mcl-1b, IAP)/(Apaf1, caspase-9) signalling; (3) damaged the tight junctions in part by down-regulating the mRNA levels ofZO-1(except spleen),ZO-2(except spleen),claudin-c,-f,-7,-11andclaudin-15, and up-regulating the mRNA levels ofclaudin-12, which were partially related to myosin light chain kinase (MLCK) signalling. Interesting, selenium deficiency failed to affect the expression ofGSTp1,Keap1a, occludin, claudin-b,claudin-3c,ZO-1(spleen only) andZO-2(spleen only) in the head kidney, spleen and skin of grass carp. Finally, based on the activities of glutathione peroxidase (GPx) and reactive oxygen species (ROS) content in the head kidney, spleen and skin, the dietary selenium requirements for young grass carp were estimated to be 0.558–0.588 mg/kg diet.
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影响因子：
5.6
作者：
Yanlei Yu;Fuming Zhang;D. Lu;Hong Zhang
通讯作者：
Yanlei Yu;Fuming Zhang;D. Lu;Hong Zhang
影响因子：
3.7
作者：
Ruocco N;Costantini S;Zupo V;Romano G;Ianora A;Fontana A;Costantini M
通讯作者：
Costantini M
苏氨酸缺乏会降低草鱼幼鱼（Ctenopharyngodon idella）感染嗜水气单胞菌后的肠道免疫力，并加重与 NF-κ B 和 i 信号通路相关的炎症
影响因子：
3.6
作者：
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通讯作者：
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影响因子：
4.7
作者：
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通讯作者：
Chenglong Wu;Jinyun Ye;Jun’e Gao;Lian Chen;Zhibin Lu
影响因子：
10.8
作者：
Roberge, Stephanie;Roussel, Julien;Fauconnier, Jeremy
通讯作者：
Fauconnier, Jeremy