Identification of podocalyxin-like protein as a high endothelial venule ligand for L-selectin: parallels to CD34.
Identification of podocalyxin-like protein as a high endothelial venule ligand for L-selectin: parallels to CD34.
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DOI：
10.1084/jem.187.12.1965
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发表时间：
1998-06-15
期刊：
影响因子：
--
通讯作者：
Rosen SD
中科院分区：
文献类型：
--
作者：
Sassetti C;Tangemann K;Singer MS;Kershaw DB;Rosen SD
白细胞粘附分子L-选择素通过与高内皮微静脉（HEV）上的特异性配体相互作用介导淋巴细胞向次级淋巴器官的募集。尽管HEV衍生的L-选择素配体仍不完全确定，但它们具有共同的唾液粘蛋白样结构，被认为将成簇的寡糖呈递到L-选择素的凝集素结构域。足糖萼蛋白样蛋白（PCLP）是一种跨膜唾液粘蛋白，其结构与已充分表征的L-选择素配体CD 34相似。PCLP在肾小球足细胞的足突和某些部位的血管内皮上均有表达。我们已经确定PCLP存在于HEV上，在那里它结合重组L-选择素和HEV特异性单克隆抗体MECA-79。此外，纯化的HEV衍生的PCLP能够支持淋巴细胞在体外生理流动条件下的束缚和滚动。这些结果表明，PCLP作为粘附分子的一种新功能，并允许在PCLP和CD 34，这可能是重要的L-选择素配体功能的保守的结构特征的定义。
The leukocyte adhesion molecule, L-selectin, mediates the recruitment of lymphocytes to secondary lymphoid organs via interactions with specific ligands presented on high endothelial venules (HEV). Although the HEV-derived ligands for L-selectin are still incompletely defined, they share a common sialomucin-like structure which is thought to present clustered oligosaccharides to the lectin domain of L-selectin. Podocalyxin-like protein (PCLP) is a transmembrane sialomucin that is similar in structure to the well-characterized L-selectin ligand CD34. PCLP has been shown previously to be expressed on the foot processes of podocytes in the kidney glomerulus as well as on vascular endothelium at some sites. We have determined that PCLP is present on HEV, where it binds to both recombinant L-selectin and the HEV-specific monoclonal antibody MECA-79. Furthermore, purified HEV-derived PCLP is able to support the tethering and rolling of lymphocytes under physiological flow conditions in vitro. These results suggest a novel function for PCLP as an adhesion molecule and allow the definition of conserved structural features in PCLP and CD34, which may be important for L-selectin ligand function.
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影响因子：
64.8
作者：
GALLATIN, WM;WEISSMAN, IL;BUTCHER, EC
通讯作者：
BUTCHER, EC
影响因子：
56.9
作者：
Butcher, EC;Picker, LJ
通讯作者：
Picker, LJ
影响因子：
6.5
作者：
Boukerche, H;RuchaudSparagano, MH;McGregor, JL
通讯作者：
McGregor, JL
L-选择蛋白的生理配体GlyCAM-1激活幼稚的外周淋巴细胞上的β2整联蛋白。
DOI：
10.1084/jem.184.4.1343
发表时间：
1996-10-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
通讯作者：
--
影响因子：
4.8
作者：
HEMMERICH, S;LEFFLER, H;ROSEN, SD
通讯作者：
ROSEN, SD