Characterization of the microbiota in the guts of Triatoma brasiliensis and Triatoma pseudomaculata infected by Trypanosoma cruzi in natural conditions using culture independent methods.
Characterization of the microbiota in the guts of Triatoma brasiliensis and Triatoma pseudomaculata infected by Trypanosoma cruzi in natural conditions using culture independent methods.
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使用培养独立的方法在自然条件下，在自然条件下，在自然条件下感染了巴西毛菌的肠道和triatoma triatoma pseudomaculata的肠道菌群的表征。
DOI：
10.1186/s13071-015-0836-z
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发表时间：
2015-04-24
影响因子：
3.2
通讯作者：
Azambuja P
中科院分区：
文献类型：
--
作者：
Gumiel M;da Mota FF;Rizzo Vde S;Sarquis O;de Castro DP;Lima MM;Garcia Ede S;Carels N;Azambuja P
恰加斯病是由克氏锥虫引起的，由锥虫病媒传播。巴西东北部地区是恰加斯病的地方病，并拥有最大的锥蝽物种多样性。克氏锥虫在其triatomine载体中的发展取决于多种因素，包括细菌肠道微生物群的组成。我们通过对细胞色素c氧化酶亚基I （COI）基因进行测序，对在俄罗斯（ceearas）市捕获的三原子虫进行了表征。采用不依赖培养的方法，通过聚合酶链式反应（PCR）、变性梯度凝胶电泳（DGGE）、DNA片段克隆、Sanger测序和454焦磷酸测序等方法扩增16S rRNA基因，研究了巴西三角瘤（Triatoma brasiliensis）和伪三角瘤（Triatoma pseudomaculata）的肠道细菌群落组成。此外，我们通过测序来自肠道宏基因组DNA的扩增子和引物，鉴定了TcI和TcII型克氏T.。通过与GenBank的COI序列比对，将家蝇生态环境中采集到的三毛螨诊断为假毛囊绦虫（T. pseudomaculata）和巴西蜱（T. brasiliensis）。克氏弓形虫在成年三角蝽中的感染率分别为80%和90%。根据DGGE条带的DNA序列，triatomine肠道菌群主要由变形菌门（Proteobacteria）和放线菌门（Actinobacteria）组成。然而，厚壁菌门和拟杆菌门也被检测到，尽管比例要低得多。沙雷氏菌是主要属，在DGGE和454焦磷酸测序分析的所有样品中都发现了沙雷氏菌。线状杆菌是放线菌的一个亚目，它的成员构成了下一个最重要的群体。16S rRNA基因的克隆和测序证实了粘质沙雷氏菌、迪茨氏菌、地戈登菌、静止棒状杆菌和谷氨酸棒状杆菌的存在。triatomine肠道细菌微生物群的研究受到越来越多的关注，因为它可能通过与T. cruzi竞争而在恰加斯病的流行病学中发挥作用。不依赖培养的方法表明，圈养三脚鼠肠道微生物群的细菌组成仅由少数几种细菌组成。本文的在线版本（doi:10.1186/s13071-015-0836-z）包含补充材料，可供授权用户使用。
Chagas disease is caused by Trypanosoma cruzi, which is transmitted by triatomine vectors. The northeastern region of Brazil is endemic for Chagas disease and has the largest diversity of triatomine species. T. cruzi development in its triatomine vector depends on diverse factors, including the composition of bacterial gut microbiota. We characterized the triatomines captured in the municipality of Russas (Ceará) by sequencing the cytochrome c oxidase subunit I (COI) gene. The composition of the bacterial community in the gut of peridomestic Triatoma brasiliensis and Triatoma pseudomaculata was investigated using culture independent methods based on the amplification of the 16S rRNA gene by polymerase chain reaction (PCR), denaturing gradient gel electrophoresis (DGGE), DNA fragment cloning, Sanger sequencing and 454 pyrosequencing. Additionally, we identified TcI and TcII types of T. cruzi by sequencing amplicons from the gut metagenomic DNA with primers for the mini-exon gene. Triatomines collected in the peridomestic ecotopes were diagnosed as T. pseudomaculata and T. brasiliensis by comparing their COI sequence with GenBank. The rate of infection by T. cruzi in adult triatomines reached 80% for T. pseudomaculata and 90% for T. brasiliensis. According to the DNA sequences from the DGGE bands, the triatomine gut microbiota was primarily composed of Proteobacteria and Actinobacteria. However, Firmicutes and Bacteroidetes were also detected, although in much lower proportions. Serratia was the main genus, as it was encountered in all samples analyzed by DGGE and 454 pyrosequencing. Members of Corynebacterinae, a suborder of the Actinomycetales, formed the next most important group. The cloning and sequencing of full-length 16S rRNA genes confirmed the presence of Serratia marcescens, Dietzia sp., Gordonia terrae, Corynebacterium stationis and Corynebacterium glutamicum. The study of the bacterial microbiota in the triatomine gut has gained increased attention because of the possible role it may play in the epidemiology of Chagas disease by competing with T. cruzi. Culture independent methods have shown that the bacterial composition of the microbiota in the guts of peridomestic triatomines is made up by only few bacterial species. The online version of this article (doi:10.1186/s13071-015-0836-z) contains supplementary material, which is available to authorized users.
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影响因子：
3.8
作者：
da Mota FF;Marinho LP;Moreira CJ;Lima MM;Mello CB;Garcia ES;Carels N;Azambuja P
通讯作者：
Azambuja P
影响因子：
3.7
作者：
Castro DP;Moraes CS;Gonzalez MS;Ratcliffe NA;Azambuja P;Garcia ES
通讯作者：
Garcia ES
影响因子：
4
作者：
Brisse, S;Verhoef, J;Tibayrenc, M
通讯作者：
Tibayrenc, M
影响因子：
4
作者：
Beard, CB;Dotson, EM;Durvasula, RV
通讯作者：
Durvasula, RV
影响因子：
3.7
作者：
Andersson, Anders F.;Lindberg, Mathilda;Jakobsson, Hedvig;Backhed, Fredrik;Nyren, Pal;Engstrand, Lars
通讯作者：
Engstrand, Lars