Trypanosoma cruzi immune response modulation decreases microbiota in Rhodnius prolixus gut and is crucial for parasite survival and development.
Trypanosoma cruzi immune response modulation decreases microbiota in Rhodnius prolixus gut and is crucial for parasite survival and development.
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DOI：
10.1371/journal.pone.0036591
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Garcia ES
中科院分区：
文献类型：
--
作者：
Castro DP;Moraes CS;Gonzalez MS;Ratcliffe NA;Azambuja P;Garcia ES
克氏锥虫为了在长尾红鳟消化道内完成发育，需要克服肠道内的免疫反应和微生物群对锥虫的水解活性。我们证明，与未感染的对照昆虫相比，感染克氏锥虫克隆Dm28c的外生马牙根虫感染后，长尾毛鼠的中肠含有(i)更少的细菌，（ii）更高的寄生虫数量，（iii）亚硝酸盐和硝酸盐产量减少，酚氧化酶和抗菌活性增加。此外，在抗生素预处理后再感染Dm28c的昆虫中，与单独感染寄生虫的昆虫相比，细菌数量减少，寄生虫载量更高。此外，与感染Dm28c的昆虫相比，感染克氏t型虫Y株导致肠道细菌数量略有减少，但不足以介导成功的寄生虫感染。我们认为，克氏T. Dm28c克隆感染长尾螺旋体后，会改变宿主肠道的免疫反应，从而减少肠道内的微生物群，这些变化对寄生虫在昆虫肠道内的发育至关重要。
Trypanosoma cruzi in order to complete its development in the digestive tract of Rhodnius prolixus needs to overcome the immune reactions and microbiota trypanolytic activity of the gut. We demonstrate that in R. prolixus following infection with epimastigotes of Trypanosoma cruzi clone Dm28c and, in comparison with uninfected control insects, the midgut contained (i) fewer bacteria, (ii) higher parasite numbers, and (iii) reduced nitrite and nitrate production and increased phenoloxidase and antibacterial activities. In addition, in insects pre-treated with antibiotic and then infected with Dm28c, there were also reduced bacteria numbers and a higher parasite load compared with insects solely infected with parasites. Furthermore, and in contrast to insects infected with Dm28c, infection with T. cruzi Y strain resulted in a slight decreased numbers of gut bacteria but not sufficient to mediate a successful parasite infection. We conclude that infection of R. prolixus with the T. cruzi Dm28c clone modifies the host gut immune responses to decrease the microbiota population and these changes are crucial for the parasite development in the insect gut.
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影响因子：
2.1
作者：
Gonzalez, MS;Nogueira, NFS;Garcia, ES
通讯作者：
Garcia, ES
DOI：
10.1590/s0074-02762007005000092
发表时间：
2007-10-01
期刊：
Memórias do Instituto Oswaldo Cruz
影响因子：
--
作者：
Dias, João Carlos Pinto
通讯作者：
Dias, João Carlos Pinto
影响因子：
5.8
作者：
Bexfield, A;Nigam, Y;Ratcliffe, NA
通讯作者：
Ratcliffe, NA
影响因子：
2.1
作者：
Eichler, S;Schaub, GA
通讯作者：
Schaub, GA
影响因子：
2.2
作者：
Genta, F. A.;Souza, R. S.;Azambuja, P.
通讯作者：
Azambuja, P.