Standard inocula preparations reduce the bacterial diversity and reliability of regulatory biodegradation tests.
Standard inocula preparations reduce the bacterial diversity and reliability of regulatory biodegradation tests.
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DOI：
10.1007/s11356-013-2064-4
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Davenport RJ
中科院分区：
文献类型：
--
作者：
Goodhead AK;Head IM;Snape JR;Davenport RJ
几十年来，经合组织的生物降解性测试一直是从监管角度了解化学品生物降解的核心。然而，由于使用的接种物浓度低、持续时间短和试验之间的变异性高，它们不适合当代基于持久性的化学品优先级排序。研究了两种OECD标准的接种物预处理方法（沉降和过滤）对污水处理厂活性污泥工艺中接种物生物降解的可能性以及相关的细菌群落结构和多样性变化的影响。与使用未处理的接种物相比，沉降和过滤都显示出显著降低4-硝基苯酚生物降解的可能性并增加其可变性。这些差异与细胞数量和固体含量显着减少了100倍，细菌群落结构的显着转变，有时伴随着显着减少可检测的操作分类单元的丰富性和均匀性。自然变化（不同环境之间）和差异选择的细菌群落（通过不同的预处理方法）的变化提供了一个解释的历史高变异性标准经合组织准备的生物降解性试验。本文的在线版本（doi：10.1007/s11356-013-2064-4）包含补充材料，可供授权用户使用。
OECD ready biodegradability tests have been central to understanding the biodegradation of chemicals from a regulatory perspective for many decades. They are not fit for contemporary prioritisation of chemicals based on persistence, however, due to the low concentration of inocula used, short duration and high variability between tests. Two OECD standard inoculum pretreatment methods (settlement and filtration) were investigated to observe their effect on the probability of biodegradation and associated changes in bacterial community structure and diversity of inocula sourced from the activated sludge process of wastewater treatment plants. Both settlement and filtration were shown to dramatically and significantly reduce the probability and increase the variability of biodegradation of 4-nitrophenol compared to the use of unprocessed inocula. These differences were associated with a significant hundred-fold reduction in cell numbers and solids content and a significant shift in bacterial community structure that was sometimes accompanied by significant reductions in detectable operational taxonomic unit richness and evenness. The natural variation (between different environments) and variation due to differential selection of bacterial communities (by different pretreatment methods) is offered as an explanation for the historical high variability in standard OECD ready biodegradability tests. The online version of this article (doi:10.1007/s11356-013-2064-4) contains supplementary material, which is available to authorized users.
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DOI：
10.1038/ismej.2012.8
发表时间：
2012-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
影响因子：
4.1
作者：
BOATMAN, RJ;CUNNINGHAM, SL;ZIEGLER, DA
通讯作者：
ZIEGLER, DA
影响因子：
6.8
作者：
GERIKE, P;FISCHER, WK
通讯作者：
FISCHER, WK
影响因子：
6.8
作者：
GERIKE, P;FISCHER, WK
通讯作者：
FISCHER, WK
影响因子：
6.8
作者：
Forney, LJ;Liu, WT;Larson, RJ
通讯作者：
Larson, RJ