Modeling the effect of collagen fibril alignment on ligament mechanical behavior.
Modeling the effect of collagen fibril alignment on ligament mechanical behavior.
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DOI：
10.1007/s10237-017-0977-4
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发表时间：
2018-04
影响因子：
3.5
通讯作者：
Lujan TJ
中科院分区：
文献类型：
--
作者：
Stender CJ;Rust E;Martin PT;Neumann EE;Brown RJ;Lujan TJ
韧带的力学行为主要由I型胶原的纤维网络调节。虽然这些纤维网络通常高度对齐，但健康和受伤的韧带也可以表现出混乱的胶原结构。本研究的目的是确定相邻韧带之间胶原纤维网络的变化是否可以预测观察到的力学行为差异。韧带标本从两个地区的牛球节关节，表现出高度一致或混乱的胶原纤维网络，在单轴拉伸力学测试。共聚焦显微镜和FiberFit软件用于量化胶原纤维分散和平均原纤维取向的机械测试标本。这两个结构参数作为输入到一个既定的超弹性本构模型，占平面原纤取向的连续分布。模型预测相邻韧带之间的机械行为差异的能力通过以下方式进行测试：1）将模型参数曲线拟合到具有高度对齐的原纤维的韧带的应力响应，然后2）使用该模型通过仅改变原纤维分散和平均原纤维取向的参数值来预测具有无序原纤维的韧带的应力响应。本研究发现，当使用基于共聚焦成像数据的原纤维分散和平均原纤维取向的参数值时，该模型强烈预测了具有无序原纤维的韧带的平均应力响应（R2=0.97）;然而，该模型仅成功预测了一半测试样本中具有无序原纤维的韧带的个体应力响应。模型预测变得更糟时，原纤维分散和平均原纤维取向参数不基于共聚焦成像数据。这些发现强调了胶原纤维排列在韧带力学中的重要性，并有助于推进对健康和受伤韧带中纤维网络的机械理解。
Ligament mechanical behavior is primarily regulated by fibrous networks of type I collagen. Although these fibrous networks are typically highly aligned, healthy and injured ligament can also exhibit disorganized collagen architecture. The objective of this study was to determine whether variations in the collagen fibril network between neighboring ligaments can predict observed differences in mechanical behavior. Ligament specimens from two regions of bovine fetlock joints, which either exhibited highly aligned or disorganized collagen fibril networks, were mechanically tested in uniaxial tension. Confocal microscopy and FiberFit software were used to quantify the collagen fibril dispersion and mean fibril orientation in the mechanically tested specimens. These two structural parameters served as inputs into an established hyperelastic constitutive model that accounts for a continuous distribution of planar fibril orientations. The ability of the model to predict differences in the mechanical behavior between neighboring ligaments was tested by 1) curve fitting the model parameters to the stress response of the ligament with highly aligned fibrils and then 2) using this model to predict the stress response of the ligament with disorganized fibrils by only changing the parameter values for fibril dispersion and mean fibril orientation. This study found that when using parameter values for fibril dispersion and mean fibril orientation based on confocal imaging data, the model strongly predicted the average stress response of ligaments with disorganized fibrils (R2=0.97); however, the model only successfully predicted the individual stress response of ligaments with disorganized fibrils in half the specimens tested. Model predictions became worse when parameters for fibril dispersion and mean fibril orientation were not based on confocal imaging data. These findings emphasize the importance of collagen fibril alignment in ligament mechanics, and help advance a mechanistic understanding of fibrillar networks in healthy and injured ligament.
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影响因子：
2.8
作者：
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通讯作者：
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影响因子：
2.9
作者：
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通讯作者：
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