Effect of elastin digestion on the quasi-static tensile response of medial collateral ligament.
Effect of elastin digestion on the quasi-static tensile response of medial collateral ligament.
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DOI：
10.1002/jor.22352
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发表时间：
2013-08
影响因子：
2.8
通讯作者：
Weiss, Jeffrey A.
中科院分区：
文献类型：
--
作者：
Henninger, Heath B.;Underwood, Clayton J.;Romney, Steven J.;Davis, Grant L.;Weiss, Jeffrey A.
弹性蛋白是一种结构蛋白，为生物组织提供弹性。我们研究了弹性蛋白的贡献，准静态拉伸反应的猪内侧副韧带，通过有针对性地破坏弹性蛋白网络与胰腺弹性蛋白酶。改变弹性蛋白酶浓度和处理时间以确定剂量反应。而弹性蛋白含量随着弹性蛋白酶浓度和处理时间的增加而降低，在循环负荷后峰值应力的变化在1 U/ml弹性蛋白酶和6小时处理后达到平台。对于用2U/ml弹性蛋白酶处理6小时的标本，弹性蛋白含量降低约35%。循环载荷后的平均峰值组织应变（4.8%，p ≥ 0.300）、模量（275 MPa，p ≥ 0.114）和滞后（20%，p ≥ 0.553）不受弹性蛋白酶消化的影响，但处理后应力显著降低（高达2 MPa，p ≤ 0.049）。弹性蛋白降解对破坏性能没有影响，但在相同的预应力下，组织延长。弹性蛋白酶消化不影响线性区域的硬度，表明酶处理不会破坏胶原蛋白。这些结果表明，弹性蛋白主要在应力-应变曲线的趾部区域中起作用，但在线性区域中有助于负载支撑。弹性蛋白酶消化后长度的增加表明弹性蛋白可以预加应力并稳定韧带中的胶原卷曲。
Elastin is a structural protein that provides resilience to biological tissues. We examined the contributions of elastin to the quasi-static tensile response of porcine medial collateral ligament through targeted disruption of the elastin network with pancreatic elastase. Elastase concentration and treatment time were varied to determine a dose response. Whereas elastin content decreased with increasing elastase concentration and treatment time, the change in peak stress after cyclic loading reached a plateau above 1 U/ml elastase and 6 hr treatment. For specimens treated with 2 U/ml elastase for 6 hr, elastin content decreased approximately 35%. Mean peak tissue strain after cyclic loading (4.8%, p≥0.300), modulus (275 MPa, p≥0.114) and hysteresis (20%, p≥0.553) were unaffected by elastase digestion, but stress decreased significantly after treatment (up to 2 MPa, p≤0.049). Elastin degradation had no effect on failure properties, but tissue lengthened under the same pre-stress. Stiffness in the linear region was unaffected by elastase digestion, suggesting that enzyme treatment did not disrupt collagen. These results demonstrate that elastin primarily functions in the toe region of the stress-strain curve, yet contributes load support in the linear region. The increase in length after elastase digestion suggests that elastin may pre-stress and stabilize collagen crimp in ligaments.
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DOI：
10.1115/1.1427698
发表时间：
2002-02-01
影响因子：
1.7
作者：
Hansen, KA;Weiss, JA;Barton, JK
通讯作者：
Barton, JK
影响因子：
3.3
作者：
Provenzano, PP;Heisey, D;Vanderby, R
通讯作者：
Vanderby, R
影响因子：
6.9
作者：
Ronchetti, IP;Alessandrini, A;Valdrè, U
通讯作者：
Valdrè, U
影响因子：
4.1
作者：
HALL, DA;CZERKAWSKI, JW
通讯作者：
CZERKAWSKI, JW
影响因子：
4.1
作者：
WOOD, GC
通讯作者：
WOOD, GC