Uptake pathways of anionic and cationic photosensitizers into bacteria.
Uptake pathways of anionic and cationic photosensitizers into bacteria.
复制标题
DOI：
10.1039/b809624d
复制
发表时间：
2009-06
期刊：
影响因子：
--
通讯作者：
Kishen A
中科院分区：
文献类型：
--
作者：
George S;Hamblin MR;Kishen A
研究了二价阳离子（钙、镁）和一种渗透剂（EDTA）对革兰氏阳性粪肠球菌和革兰氏阴性放线菌comitans吸收阳离子光敏剂（PS）亚甲基蓝（MB）和两种阴离子光敏剂玫瑰红（RB）和吲哚菁绿（ICG）的影响。通过分别使用外排泵抑制剂（维拉帕米）和胰蛋白酶处理的研究，评估了多药物外排泵和蛋白质转运体在粪肠球菌细胞中光敏剂摄取中的可能作用。二价阳离子增强了粪肠杆菌和放线菌comitans对RB和ICG的摄取和光动力失活电位，同时降低了MB的摄取和光动力失活电位。Verapamil增加了RB的摄取（可能是由于外排泵抑制），而胰蛋白酶处理导致RB和ICG的摄取显著降低。结果表明，细菌细胞对阴离子PSs的摄取可能是通过静电相互作用和蛋白质转运体的结合来介导的，而阳离子PSs的摄取，如先前报道的那样，是通过静电相互作用和自促进摄取途径来介导的。
The effect of divalent cations (calcium and magnesium) and a permeabilizing agent (EDTA) on the uptake of a cationic photosensitizer (PS), methylene blue (MB), and two anionic PSs, rose bengal (RB) and indocyanine green (ICG), by Gram-positive Enterococcus faecalis and Gram-negative Actinobacillus actinomycetemcomitans was examined. The possible roles of multidrug efflux pumps and protein transporters in photosensitizer uptake were assessed in E. faecalis cells by studies using an efflux pump inhibitor (verapamil) and trypsin treatment respectively. Divalent cations enhanced the uptake and photodynamic inactivation potential of both RB and ICG in E. faecalis and A. actinomycetemcomitans, while they decreased the uptake and bacterial killing by MB. Verapamil increased the uptake of RB (possibly due to efflux pump inhibition), whereas trypsin treatment resulted in significant decrease in RB and ICG uptake. The results suggested that the uptake of anionic PSs by bacterial cells may be mediated through a combination of electrostatic charge interaction and by protein transporters, while the uptake of cationic PSs, as previously reported, is mediated by electrostatic interactions and self promoted uptake pathways.
登录
查看更多内容
DOI：
10.1016/s1011-1344(96)07453-2
发表时间：
1997-04-01
影响因子：
5.4
作者：
Fickweiler, S;Szeimies, RM;Landthaler, M
通讯作者：
Landthaler, M
影响因子：
4
作者：
Lambert, PA
通讯作者：
Lambert, PA
影响因子：
4.9
作者：
Kishen, A;George, S;Kumar, R
通讯作者：
Kumar, R
影响因子：
5.2
作者：
Hamblin, MR;O'Donnell, DA;Hasan, T
通讯作者：
Hasan, T
影响因子：
3.3
作者：
Bhatti, M;MacRobert, A;Wilson, M
通讯作者：
Wilson, M