Fast photocurable thiol-ene elastomers with tunable biodegradability, mechanical and surface properties enhance myoblast differentiation and contractile function.
Fast photocurable thiol-ene elastomers with tunable biodegradability, mechanical and surface properties enhance myoblast differentiation and contractile function.
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DOI：
10.1016/j.bioactmat.2020.12.022
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发表时间：
2021-07
影响因子：
18.9
通讯作者：
Cheng C
中科院分区：
文献类型：
--
作者：
Mohamed MA;Shahini A;Rajabian N;Caserto J;El-Sokkary AMA;Akl MA;Andreadis ST;Cheng C
生物可降解弹性体是重要的新兴生物材料，用于生物医学应用，特别是在软组织工程领域，其中支架需要匹配天然组织的物理化学性质。在这里，我们报告新的快速光固化弹性体，具有容易调节的机械性能，表面润湿性和降解性。这些弹性体是通过对甘油三戊烯酸酯（GTP;一种三烯）或GTP和4-戊烯基4-戊烯酸酯（PP;一种二烯）的组合与精心选择的一系列二硫醇或三硫醇的硫醇-烯反应体系进行5分钟紫外线照射来制备的。在随后的应用研究中，发现这些弹性体能够克服肌管的分层，这是限制成熟功能性肌纤维体外生长的技术瓶颈。甘油基弹性体支持小鼠和人类成肌细胞的增殖，以及成肌分化为收缩性肌管。更值得注意的是，当从常规组织培养板上分离的小鼠肌管跳动时，它们仍然粘附在弹性体表面上。结果表明，这些弹性体作为新型生物材料可能提供一个有前途的平台，工程功能的软组织在再生医学或药理学测试的潜在应用。通过硫醇-烯UV固化在5分钟内形成生物相容性弹性体。弹性体的可调润湿性、机械性能和降解性。弹性体能显著促进成肌细胞的肌源性分化。弹性体上的肌管表现出增强的附着和跳动频率。
Biodegradable elastomers are important emerging biomaterials for biomedical applications, particularly in the area of soft-tissue engineering in which scaffolds need to match the physicochemical properties of native tissues. Here, we report novel fast photocurable elastomers with readily tunable mechanical properties, surface wettability, and degradability. These elastomers are prepared by a 5-min UV-irradiation of thiol-ene reaction systems of glycerol tripentenoate (GTP; a triene) or the combination of GTP and 4-pentenyl 4-pentenoate (PP; a diene) with a carefully chosen series of di- or tri-thiols. In the subsequent application study, these elastomers were found to be capable of overcoming delamination of myotubes, a technical bottleneck limiting the in vitro growth of mature functional myofibers. The glycerol-based elastomers supported the proliferation of mouse and human myoblasts, as well as myogenic differentiation into contractile myotubes. More notably, while beating mouse myotubes detached from conventional tissue culture plates, they remain adherent on the elastomer surface. The results suggest that these elastomers as novel biomaterials may provide a promising platform for engineering functional soft tissues with potential applications in regenerative medicine or pharmacological testing. Biocompatible elastomers are formed by thiol-ene UV-curing within 5 min. Tunable wettability, mechanical properties, and degradability of the elastomers. The elastomers remarkably promote myogenic differentiation of myoblasts. Myotubes on the elastomers exhibit enhanced attachment and beating frequency.
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影响因子：
18.9
作者：
Gyawali D;Kim JP;Yang J
通讯作者：
Yang J
DOI：
10.1590/s0100-879x2004001200007
发表时间：
2004-12-01
影响因子：
2.3
作者：
Costa, M.L.;Escaleira, R.;Mermelstein, C.S.
通讯作者：
Mermelstein, C.S.
影响因子：
3.4
作者：
Engler, A;Bacakova, L;Discher, D
通讯作者：
Discher, D
影响因子：
4.9
作者：
Browe, Daniel;Freeman, Joseph
通讯作者：
Freeman, Joseph
DOI：
10.1083/jcb.200405004
发表时间：
2004-09-13
期刊：
The Journal of cell biology
影响因子：
--
作者：
Engler AJ;Griffin MA;Sen S;Bönnemann CG;Sweeney HL;Discher DE
通讯作者：
Discher DE