Persistent sodium conductance contributes to orexin-A-mediated modulation of membrane excitability in neonatal rat mesencephalic V neurons
Persistent sodium conductance contributes to orexin-A-mediated modulation of membrane excitability in neonatal rat mesencephalic V neurons
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持续钠电导有助于食欲素 A 介导的新生大鼠中脑 V 神经元膜兴奋性调节
DOI：
10.1016/j.neulet.2021.135846
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发表时间：
2021
影响因子：
2.5
通讯作者：
Mikihiko Kogo
中科院分区：
文献类型：
--
作者：
Susumu Tanaka;Soju Seki;Yudai Ono;Akifumi Enomoto;Mikihiko Kogo
食欲素是一种多功能的下丘脑神经肽，参与刺激摄食行为和能量消耗。然而，很少有人知道他们的神经调节作用，在较低的脑干效应区，包括在三叉神经元系统。本研究的目的是研究食欲素-A（Ox-A）对中脑三叉神经（Mes V）神经元的膜特性的影响，这些神经元在有节奏的口腔运动活动的产生和控制中起着至关重要的作用。从Sprague-Dawley大鼠（出生后第12-17天）制备的冠状脑切片中的Mes V神经元获得全细胞膜片钳记录。浴应用Ox-A（100 nM）缩短了动作电位后超极化的持续时间，而重复放电过程中放电的峰间频率增加，同时频率-电流关系向左偏移。此外，Ox-A放大了去极化膜电位处的共振，并伴随着Q值和共振频率的增加。进一步的电压钳实验表明Ox-A增加持续钠电流（INaP）的峰值电流密度，使其激活曲线向超极化方向移动。这些结果提示Ox-A可能通过增强INaP而增强Mes V神经元的兴奋性，其机制可能与另一种促食欲的下丘脑神经肽神经肽Y相同。
Orexins are multifunctional hypothalamic neuropeptides that participate in the stimulation of feeding behavior and energy expenditure. However, little is known about their neuromodulatory effects in lower brainstem effector regions, including in the trigeminal neuronal system. The aim of this study was to examine the effects of orexin-A (Ox-A) on the membrane properties of mesencephalic trigeminal (Mes V) neurons that are critically involved in the generation and control of rhythmical oral motor activities. Whole-cell patch clamp recordings were obtained from Mes V neurons in coronal brain slices prepared from Sprague-Dawley rats (postnatal day 12–17). Bath application of Ox-A (100 nM) shortened the duration of the after-hyperpolarization following the action potential, while the interspike frequency of firings during repetitive discharge increased, together with a shift in the frequency-current relationship toward the left. In addition, Ox-A amplified the resonance at the depolarized membrane potential, accompanied with an increase in both Q-value and resonant frequency. A further voltage-clamp experiment demonstrated that Ox-A increased the peak current density of the persistent sodium current (INaP) and shifted its activation curve to the hyperpolarization direction. These results suggested that Ox-A may increase Mes V neuronal excitability by enhancing INaP, possibly sharing a common mechanism with another orexigenic hypothalamic neuropeptide, neuropeptide Y.
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影响因子：
4.8
作者：
Baird, John-Paul;Choe, Angela;Grigg, Lindsay A.
通讯作者：
Grigg, Lindsay A.
影响因子：
2.5
作者：
Susumu Tanaka;Nanping Wu;C. Hsaio;J. Turman;S. H. Chandler
通讯作者：
Susumu Tanaka;Nanping Wu;C. Hsaio;J. Turman;S. H. Chandler
影响因子：
2.9
作者：
Nambu, T;Sakurai, T;Goto, K
通讯作者：
Goto, K
影响因子：
2.5
作者：
Peever, JH;Lai, YY;Siegel, JM
通讯作者：
Siegel, JM
影响因子：
2.5
作者：
Wu, NP;Enomoto, A;Chandler, SH
通讯作者：
Chandler, SH