Proteomic analysis of optic nerve lipid rafts reveals new paranodal proteins.
Proteomic analysis of optic nerve lipid rafts reveals new paranodal proteins.
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DOI：
10.1002/jnr.21984
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发表时间：
2009-11-15
影响因子：
4.2
通讯作者：
Rasband, Matthew N.
中科院分区：
文献类型：
--
作者：
Ogawa, Yasuhiro;Rasband, Matthew N.
节点旁连接处的神经元-胶质细胞相互作用在动作电位传播中发挥重要作用。在它们的众多功能中，它们有助于有髓神经纤维的被动电特性，并主动调节沿轴突的离子通道的极化分布。尽管它们很重要，但人们对负责副节点形成和功能的分子知之甚少。节点旁连接的形成显然取决于三种细胞粘附分子之间的相互作用：轴突上的 caspr 和 contactin，以及神经胶质膜上的神经成束蛋白 155 (NF-155)。使用Caspr无效的节点旁突变小鼠，我们证明节点旁连接的丢失导致NF-155无法分配到脂筏中，这表明位于节点旁连接处的蛋白质具有脂筏相关蛋白的生化特征。基于节点旁连接的这一特性，我们使用从纯白质束（视神经）分离的脂筏的质谱来寻找新的节点旁蛋白。由于我们使用了相对粗糙的生化制剂，因此我们鉴定了数百种不同的蛋白质。其中，我们发现了所有先前描述的旁节蛋白。基于中枢和外周神经抗体染色的进一步分析表明，β-内收蛋白、septin 2 和 sh3p8 是假定的旁节蛋白。我们描述了这些蛋白质相对于成人和发育神经纤维中的节点、旁节和旁节旁其他标记物的定位。最后，我们描述了它们在髓鞘形成障碍 TremblerJ 小鼠（周围神经病夏科-玛丽-图思病的模型）中的分布。
Neuron-glia interactions at paranodal junctions play important roles in action potential propagation. Among their many functions, they contribute to the passive electrical properties of myelinated nerve fibers and actively regulate the polarized distribution of ion channels along axons. Despite their importance, relatively little is known about the molecules responsible for paranode formation and function. Paranodal junction formation apparently depends on interactions among three cell adhesion molecules: caspr and contactin on the axon, and neurofascin 155 (NF-155) on the glial membrane. Using Caspr-null paranodal mutant mice we demonstrate that loss of paranodal junctions causes failure of NF-155 to partition into lipid rafts, indicating that proteins located at paranodal junctions have biochemical characteristics of lipid raft associated proteins. Based on this property of paranodal junctions, we used mass-spectrometry of lipid rafts isolated from a pure white matter tract (optic nerve) to search for new paranodal proteins. Since we used a relatively crude biochemical preparation, we identified several hundred different proteins. Among these, we found all previously described paranodal proteins. Further analysis based on antibody staining of central and peripheral nerves revealed β-adducin, septin 2, and sh3p8 as putative paranodal proteins. We describe the localization of these proteins in relation to other markers of nodes, paranodes, and juxtaparanodes in adult and developing nerve fibers. Finally, we describe their distribution in dysmyelinating TremblerJ mice, a model for the peripheral neuropathy Charcot-Marie-Tooth disease.
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DOI：
10.1083/jcb.200207050
发表时间：
2002-10-28
期刊：
The Journal of cell biology
影响因子：
--
作者：
Poliak S;Matlis S;Ullmer C;Scherer SS;Peles E
通讯作者：
Peles E
影响因子：
3.3
作者：
Abdi, Khadar M.;Bennett, Vann
通讯作者：
Bennett, Vann
影响因子：
5.3
作者：
Rasband, MN;Peles, E;Shrager, P
通讯作者：
Shrager, P
影响因子：
4.2
作者：
Ohno, Nobuhiko;Terada, Nobuo;Ohno, Shinichi
通讯作者：
Ohno, Shinichi
影响因子：
5.3
作者：
Rios, JC;Rubin, M;Salzer, JL
通讯作者：
Salzer, JL