INSIGHTS INTO THE ROLES OF NON-MUSCLE MYOSIN IIA IN HUMAN KERATINOCYTE MIGRATION.
INSIGHTS INTO THE ROLES OF NON-MUSCLE MYOSIN IIA IN HUMAN KERATINOCYTE MIGRATION.
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DOI：
10.1007/s12195-009-0094-2
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发表时间：
2009-11-21
影响因子：
2.8
通讯作者：
Baskaran, Harihara
中科院分区：
文献类型：
--
作者：
Sarkar, Saheli;Egelhoff, Thomas;Baskaran, Harihara
关键词：
表皮细胞迁移是伤口愈合反应中的关键因素，由F-肌动蛋白-肌球蛋白II系统调节。以前的报道已经确定了非肌肉肌球蛋白II（NMII）在调节细胞迁移中的重要性。然而，NMII在原代人角质形成细胞中的作用尚未研究。在这项研究中，我们使用了基于微加工的二维迁移试验，以检查角质形成细胞迁移中的NMII的作用。我们开发了各种尺寸（0.025 - 0.25 mm 2）的融合细胞岛，并将迁移定量为岛面积随时间的倍数增加。我们在这里报告，匪II表达和激活迁移角质形成细胞。与对照相比，用blebbistatin抑制NMIIA运动活性在6小时内显著增加了所有细胞岛大小的迁移。通过Y-27632抑制Rho激酶并不改变迁移，而通过ML-7抑制肌球蛋白轻链激酶在6小时内显著抑制迁移。blebbistatin和Y-27632均诱导形成大的膜皱褶和细长的尾部。相比之下，ML-7阻断细胞铺展，导致圆形形态。总之，这些数据表明，NMIIA减少角质形成细胞的迁移，但该机制可能是由上游激酶差异调节。
Epidermal cell migration is a key factor in wound healing responses, regulated by the F-actin-myosin II systems. Previous reports have established the importance of non-muscle myosin II (NMII) in regulating cell migration. However, the role of NMII in primary human keratinocytes has not been investigated. In this study we used a microfabrication-based two-dimensional migration assay to examine the role of NMII in keratinocyte migration. We developed confluent cell islands of various sizes (0.025 – 0.25 mm2) and quantified migration as Fold Increase in island area over time. We report here that NMII was expressed and activated in migrating keratinocytes. Inhibition of NMIIA motor activity with blebbistatin increased migration significantly in all cell island sizes in six hours compared to control. Inhibition of Rho-kinase by Y-27632 did not alter migration while inhibition of myosin light chain kinase by ML-7 suppressed migration significantly in six hours. Both blebbistatin and Y-27632 induced formation of large membrane ruffles and elongated tails. In contrast, ML-7 blocked cell spreading, resulting in a rounded morphology. Taken together, these data suggest that NMIIA decreases migration in keratinocytes, but the mechanism may be differentially regulated by upstream kinases.
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影响因子：
4.8
作者：
Amano, M;Ito, M;Kaibuchi, K
通讯作者：
Kaibuchi, K
影响因子：
4.8
作者：
Worthylake, RA;Burridge, K
通讯作者：
Burridge, K
DOI：
10.1111/j.1432-1033.1989.tb15057.x
发表时间：
1989-10-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
KATSURAGAWA, Y;YANAGISAWA, M;MASAKI, T
通讯作者：
MASAKI, T
影响因子：
4
作者：
Conti, Mary Anne;Adelstein, Robert S.
通讯作者：
Adelstein, Robert S.
DOI：
10.1016/j.bbrc.2003.12.031
发表时间：
2004-01-23
影响因子：
3.1
作者：
Duxbury, MS;Ashley, SW;Whang, EE
通讯作者：
Whang, EE